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PMID:21239225

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Citation

Okamoto-Kainuma, A, Ishikawa, M, Nakamura, H, Fukazawa, S, Tanaka, N, Yamagami, K and Koizumi, Y (2011) Characterization of rpoH in Acetobacter pasteurianus NBRC3283. J. Biosci. Bioeng. 111:429-32

Abstract

The RpoH in Acetobacter pasteurianus NBRC3283 was characterized. It was revealed that the rpoH controls the expression of groEL, dnaKJ, grpE, and clpB to different extents. In addition, the rpoH disruption mutant became apt to be affected by heat, ethanol, and acetic acid, indicating its importance in acetic acid fermentation.

Links

PubMed Online version:10.1016/j.jbiosc.2010.12.016

Keywords

Acetic Acid/metabolism; Acetobacter/genetics; Acetobacter/growth & development; Acetobacter/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Ethanol/metabolism; Fermentation; Heat-Shock Proteins/genetics; Heat-Shock Proteins/physiology; Hot Temperature; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Sigma Factor/genetics; Sigma Factor/physiology; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ACEPA:C7K2B1

GO:0003899: DNA-directed RNA polymerase activity

IMP: Inferred from Mutant Phenotype:

F

Fig. 1 shows a decreased production of chaperones after heat shock in rpoH mutant strain compared to parental strain.

complete
CACAO 8870


ACEPA:C7K2B1

GO:0016987: sigma factor activity

IMP: Inferred from Mutant Phenotype:

F

Fig. 3 demonstrates that rpoH mutants have significantly decreased survival when presented with heat, ethanol, and acid stress.

complete
CACAO 8872


See also

References

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