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User:Hylandth

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesCANAL:Q5AJV52014-04-03 11:24:07 CDTGO:0042710 biofilm formation (P)PMID:24612417ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7 shows that mutants with deletions in FGR23 has less sexual aggregation than the wild type

challenge
updatedbyinstructorARATH:PLAS22014-04-03 12:12:57 CDTGO:0046028 electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity (F)PMID:12773541ECO:0000316 genetic interaction evidence used in manual assertion

Figure 2 shows that mutants that lack plastocyanin fluoresce less with fluorescence spectroscopy

challenge
acceptableHUMAN:TLR92014-04-05 10:29:35 CDTGO:0032640 tumor necrosis factor production (P)PMID:23826189ECO:0000314 direct assay evidence used in manual assertion

Fiugre 1:Two samples of mononuclear cells were collected, TLR 9 agonist was added in one sample, and absent in another. Levels of TNF Alpha were measured respectively and seen higher when induced with the agonist.

challenge
acceptableHUMAN:TLR22014-04-05 10:37:20 CDTGO:0001819 interleukin-10 production (P)PMID:23826189ECO:0000314 direct assay evidence used in manual assertion

Figure 2A

challenge
updatedbyinstructorHUMAN:A1AG12014-04-05 11:02:45 CDTGO:0032715 negative regulation of interleukin-6 production (P)PMID:23973664ECO:0000314 direct assay evidence used in manual assertion

Figure 8: Transcript analysis from cells with ORM 1 stimulation vs cells that are not ORM 1 stimulated. Less interleukin 6 transcript is made in the with ORM 1 stimulation.

challenge
acceptableHUMAN:A1AG12014-04-05 11:07:35 CDTGO:0032720 negative regulation of tumor necrosis factor production (P)PMID:23973664ECO:0000314 direct assay evidence used in manual assertion

Figure 8: Cells that are treated with ORM 1 stimulant for 48 hours or longer produce less TNF alpha than when no stimulation of ORM 1 is induced.

challenge
unacceptableARATH:MKP12014-04-05 12:08:30 CDTGO:0052258 negative regulation by organism of pathogen-associated molecular pattern-induced innate immune response of other organism involved in symbiotic interaction (P)PMID:21447069ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2: Plants with mkp1 die when introduced to PAMP elf 26, while WT do not exude the same phenotype. When not treated with PAMP elf 26, all plants display the normal phenotype

challenge

acceptable:3
unacceptable:1
requires_changes:1
flagged:0

Annotations challenged by Hylandth

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorHylandth,
MichSt14A 37
ARATH:PLAS2GO:0046028 - electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity (F)PMID:12773541ECO:0000316 genetic interaction evidence used in manual assertionchallengeC: 2
requireschangesKaitlinm,
Team Rubby ducky
VIBF1:Q5E6Z4GO:0000155 - phosphorelay sensor kinase activity (F)PMID:15916958ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 3

0 annotations fixed by Hylandth

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