PMID:12773541

Citation	Weigel, M, Varotto, C, Pesaresi, P, Finazzi, G, Rappaport, F, Salamini, F and Leister, D (2003) Plastocyanin is indispensable for photosynthetic electron flow in Arabidopsis thaliana. J. Biol. Chem. 278:31286-9
Abstract	Plastocyanin is a soluble copper-containing protein present in the thylakoid lumen, which transfers electrons to photosystem I. In the chloroplast of the flowering plant Arabidopsis thaliana, a cytochrome c6-like protein is present, which was recently suggested to function as an alternative electron carrier to plastocyanin. We show that Arabidopsis plants mutated in both of the two plastocyanin-coding genes and with a functional cytochrome c6 cannot grow photoautotrophically because of a complete block in light-driven electron transport. Even increased dosage of the gene encoding the cytochrome c6-like protein cannot complement the double mutant phenotype. This demonstrates that in Arabidopsis only plastocyanin can donate electrons to photosystem I in vivo.
Links	PubMed Online version:10.1074/jbc.M302876200
Keywords	Arabidopsis/genetics; Arabidopsis/metabolism; Base Sequence; DNA Transposable Elements; Electron Transport; Mutagenesis; Photosynthesis/physiology; Photosynthetic Reaction Center Complex Proteins/metabolism; Photosystem I Protein Complex; Phylogeny; Plastocyanin/genetics; Plastocyanin/metabolism
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Significance

Gene product

Qualifier

GO Term

Evidence Code

with/from

Aspect

Extension

Notes

Status

enables

GO:0046028: electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity

ECO:0000316: genetic interaction evidence used in manual assertion

UniProtKB:P11490

F

Seeded From UniProt

complete

GO:0046028: electron transporter, transferring electrons from cytochrome b6/f complex of photosystem II activity

ECO:0000316:

UniProtKB:P11490

F

Figure 2 shows that mutants that lack plastocyanin fluoresce less with fluorescence spectroscopy

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