User:Hibbsder

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	STRTR:CAS3	2013-03-28 12:38:00 CDT	GO:0004536 deoxyribonuclease activity (F)	PMID:21343909	ECO:0000314 direct assay evidence used in manual assertion	Figure 3 (A) shows the nuclease activity of Cas3 in the presence of MG2+ ions. Incubating Cas3 with M13mp18 ssDNA over a two hour period shows increased levels of Cas3 correspond to increased ssDNA degradation.	challenge
acceptable	HUMAN:KDM5B	2013-04-13 18:34:10 CDT	GO:0032453 histone demethylase activity (H3-K4 specific) (F)	PMID:18048344	ECO:0000314 direct assay evidence used in manual assertion	figure 2 shows JARID1B removed H3K4 methylation in vivo. figure shows cell lines that have been with Myc-JARID1B, then immunostained with antibodies specific to methylated lysine residues. The top rows of A,B and C are those that are H3K4, and show cells are not visible in the red stain (the methylated lysine stain), compared to the other stain controls, indicating they have been demethylated.	challenge
requireschanges	MOUSE:HDAC1	2013-04-13 19:23:42 CDT	GO:0004407 histone deacetylase activity (F)	PMID:18501342	ECO:0000314 direct assay evidence used in manual assertion	Fig1.A shows that the staining intensity of HDAC1 are inversely proportional to that of acetylated H4K5 during oocyte maturation and reimplementation development. Fig7.A shows RNAi-mediated reduction of HDAC1 causing an increase for hyperacetylated histone H4.	challenge
updatedbyinstructor	HUMAN:P53	2013-04-14 10:33:40 CDT	GO:0045893 positive regulation of transcription, DNA-dependent (P)	PMID:20378837	ECO:0000314 direct assay evidence used in manual assertion	figure 2 show that p53 increases GLS2 transcription from 2 p53 inducible cell lines. After p53 had been induced, the levels of GLS2 mRNA increased by 35 fold in one line and 14 fold in the other as measured by real-time-PCR	challenge
acceptable	MOUSE:HDAC2	2013-04-14 10:51:52 CDT	GO:0034739 histone deacetylase activity (H4-K16 specific) (F)	PMID:23516383	ECO:0000315 mutant phenotype evidence used in manual assertion	fig4. C and D show that H4K16 acetylation is greatly increased in Hdac-/- knockouts compared to wild type in mouse oocytes. fig4.C shows Immunocytochemical detection of HDAC1 and histone H4K16 acetylation, and fig4.D shows the quantification of the data from C.	challenge

acceptable:3
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Hibbsder

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Hibbsder

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