User:Hazardb

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	BPT7:Y77	2016-04-11 04:37:27 CDT	GO:0039620 T=7 icosahedral viral capsid (C)	PMID:17040895	ECO:0000314 direct assay evidence used in manual assertion	This is a T7 capsid protein (gp10) that is used in combination with a enhanced yellow fluorescent protein (EYFP). The protein gp10 is with in a T7 phage. When combining this phage with the fluorescent protein, the phage only allows a proportion of the capsid protein to be used. In the paper they described out they made a frameshift that allowed bacterial ribosome to read the reading frame and hit a stop codon after the capsid protein. The frame then shifts to -1 and allows the bacterial ribosomes to continue reading the fluorescent protein. This creates T7 fluorescent phages that allow us to see the interaction between displayed proteins and their binding partners with in the T7 phage. The evidence is in figure 1 of the paper.	challenge
updatedbyinstructor	AQUAE:O67450	2016-04-25 09:35:54 CDT	GO:0032508 DNA duplex unwinding (P)	PMID:24373746	ECO:0000315 mutant phenotype evidence used in manual assertion	"When the dilated and constricted mutants were tested in a single-turnover, fork-unwinding assay with DnaC, the dilated mutant was able to produce ∼20% more unwound product as the wild-type helicase and did so at a slightly faster rate (Figure 5B). By contrast, while the constricted mutant could generate an amount of ssDNA comparable to that of wild-type DnaB, it took longer to do so (Figure 5B)."	challenge
unacceptable	9CAUD:A4ZFB3	2016-04-27 09:21:29 CDT	GO:0019028 viral capsid (This is the capsid protein in the bacteriophage 80alpha. In figure 3, D, it shows capsids being created with proteins starting at gp42 and ending at gp47 . This shows that this is the full capsid protein within the bacteriophage 80alpha. The protein goes on to describe how section D would not be possible if it did not contain gp47, leading to believe that this is the capsid protein. Basically if you put everything from gp42 to gp46 no capsid would form. )	PMID:22980502	IDA: Inferred from Direct Assay		challenge
updatedbyinstructor	9CAUD:A4ZFB2	2016-05-06 09:26:34 CDT	GO:0046806 viral scaffold (C)	PMID:22980502	ECO:0000315 mutant phenotype evidence used in manual assertion	As shown above, gp47 does not assemble efficiently in the absence of gp46 (Figs. 3A and 4).	challenge

acceptable:0
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Hazardb

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Corneljl, Team Phages Woo!	9CAUD:A0A024B145	GO:0034061 - DNA polymerase activity (F)	PMID:4622590	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 6
unacceptable	Natjack1, Team Green B	9CAUD:A0A0K2CZZ1	GO:0004604 - phosphoadenylyl-sulfate reductase (thioredoxin) activity (F)	GO_REF:0000100	ECO:0000250 sequence similarity evidence used in manual assertion	challenge	C: 3
unacceptable	Paradaje, Team Go Dogs Go	9CAUD:A0A024AZS4	GO:0097619 - PTEX complex (C)	PMID:23012374	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 5
unacceptable	Amariam1, Team You can't B. cereus	9CAUD:A0A0K2D089	GO:0046798 - viral portal complex (C)	PMID:18567664	ECO:0000250 sequence similarity evidence used in manual assertion	challenge	C: 3
unacceptable	Hoang5, Team Purple A	9CAUD:A0A0K2D0M7	GO:0008168 - methyltransferase activity (F)	PMID:10615135	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3

0 annotations fixed by Hazardb

What do the icons mean in the status column?