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BPT7:Y77
Contents
| Species (Taxon ID) | Enterobacteria phage T7 (Bacteriophage T7). (10760) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | Protein 7.7
Gene product 7.7 Gp7.7 | |
| External Links | ||
| UniProt | P03796 | |
| EMBL | V01146 | |
| PIR | A04421 | |
| RefSeq | NP_041994.1 | |
| ProteinModelPortal | P03796 | |
| GeneID | 1261028 | |
| KEGG | vg:1261028 | |
| Proteomes | UP000000840 | |
| InterPro | IPR003615 | |
| Pfam | PF13392 | |
| SMART | SM00507 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0039620 |
T=7 icosahedral viral capsid |
ECO:0000314 |
C |
This is a T7 capsid protein (gp10) that is used in combination with a enhanced yellow fluorescent protein (EYFP). The protein gp10 is with in a T7 phage. When combining this phage with the fluorescent protein, the phage only allows a proportion of the capsid protein to be used. In the paper they described out they made a frameshift that allowed bacterial ribosome to read the reading frame and hit a stop codon after the capsid protein. The frame then shifts to -1 and allows the bacterial ribosomes to continue reading the fluorescent protein. This creates T7 fluorescent phages that allow us to see the interaction between displayed proteins and their binding partners with in the T7 phage. The evidence is in figure 1 of the paper. |
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Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Slootweg, EJ et al. (2006) Fluorescent T7 display phages obtained by translational frameshift. Nucleic Acids Res. 34 e137 PubMed GONUTS page
