PMID:22980502

Citation	Spilman, MS, Damle, PK, Dearborn, AD, Rodenburg, CM, Chang, JR, Wall, EA, Christie, GE and Dokland, T (2012) Assembly of bacteriophage 80α capsids in a Staphylococcus aureus expression system. Virology 434:242-50
Abstract	80α is a temperate, double-stranded DNA bacteriophage of Staphylococcus aureus that can act as a "helper" for the mobilization of S. aureus pathogenicity islands (SaPIs), including SaPI1. When SaPI1 is mobilized by 80α, the SaPI genomes are packaged into capsids that are composed of phage proteins, but that are smaller than those normally formed by the phage. This size determination is dependent on SaPI1 proteins CpmA and CpmB. Here, we show that co-expression of the 80α capsid and scaffolding proteins in S. aureus, but not in E. coli, leads to the formation of procapsid-related structures, suggesting that a host co-factor is required for assembly. The capsid and scaffolding proteins also undergo normal N-terminal processing upon expression in S. aureus, implicating a host protease. We also find that SaPI1 proteins CpmA and CpmB promote the formation of small capsids upon co-expression with 80α capsid and scaffolding proteins in S. aureus.
Links	PubMed PMC3518739 Online version:10.1016/j.virol.2012.08.031
Keywords	Bacterial Proteins/metabolism; Capsid/metabolism; Escherichia coli/genetics; Gene Expression; Genetics, Microbial/methods; Genomic Islands; Humans; Molecular Biology/methods; Protein Multimerization; Staphylococcus Phages/genetics; Staphylococcus Phages/physiology; Staphylococcus aureus/genetics; Staphylococcus aureus/virology; Virology/methods; Virus Assembly
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:A4ZFB2		GO:0046806: viral scaffold	ECO:0000315: mutant phenotype evidence used in manual assertion		C		As shown above, gp47 does not assemble efficiently in the absence of gp46 (Figs. 3A and 4).	complete CACAO 11966
9CAUD:A4ZFB3		GO:0019028: viral capsid	IDA: Inferred from Direct Assay:	C	This is the capsid protein in the bacteriophage 80alpha. In figure 3, D, it shows capsids being created with proteins starting at gp42 and ending at gp47 . This shows that this is the full capsid protein within the bacteriophage 80alpha. The protein goes on to describe how section D would not be possible if it did not contain gp47, leading to believe that this is the capsid protein. Basically if you put everything from gp42 to gp46 no capsid would form.	complete CACAO 11925

References

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