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User:Findley4

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableHUMAN:ST1E12014-04-06 11:50:26 CDTGO:0045600 positive regulation of fat cell differentiation (P)PMID:24567372ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A and 2C show that preadipocytes in which SULT1E1 mRNA was overexpressed had higher rates of adipogenesis than the control preadipocytes with low levels of SULT1E1.

challenge
acceptableBACHD:Q9KFY32014-04-06 12:41:50 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:22841292ECO:0000314 direct assay evidence used in manual assertion

Figure 1C shows that Cas5d (BH0337 protein) processes pre-crRNA in vitro.

challenge
acceptableBACHD:Q9KFY32014-04-06 12:51:03 CDTGO:0004521 endoribonuclease activity (F)PMID:22841292ECO:0000314 direct assay evidence used in manual assertion

Figure 1D shows that cas5d (BH0337 protein) has endoribonuclease activity because it can cleave RNA.

challenge
acceptableBACHD:Q9KFY32014-04-06 13:06:08 CDTGO:0004518 nuclease activity (F)PMID:24371266ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that cas5d (BH0337 protein) has DNase activity on linear dsDNA, circular dsDNA, and ssDNA.

challenge
updatedbyinstructorHALVD:CAS52014-04-06 13:57:24 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:24459147ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows by complementation tests that a complex of cas5, cas6, and cas7 are necessary for crRNA processing

challenge
unacceptableHALVD:D4GQN42014-04-06 14:00:04 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:24459147ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows that a cas6 deletion mutant cannot generate crRNA.

challenge
acceptablePYRFU:CAS62014-04-06 14:45:52 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:19141480ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that cas6 cleaves RNA in repeat sequences of CRISPR elements.

challenge

acceptable:5
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Findley4

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Findley4

What do the icons mean in the status column?

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