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PMID:24567372
| Citation |
Ihunnah, CA, Wada, T, Philips, BJ, Ravuri, SK, Gibbs, RB, Kirisci, L, Rubin, JP, Marra, KG and Xie, W' (2014) Estrogen Sulfotransferase (EST/SULT1E1) Promotes Human Adipogenesis. Mol. Cell. Biol. ' |
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| Abstract |
The estrogen sulfotransferase (EST/SULT1E1) is known to catalyze the sulfoconjugation and deactivation of estrogens. The goal of this study is to determine whether and how EST plays a role in human adipogenesis. By using human primary adipose derived stem cells (ASCs) and whole fat tissues from the abdominal subcutaneous fat of obese and non-obese subjects, we showed that the expression of EST was low in pre-adipocytes but increased upon differentiation. Overexpression and knockdown of EST in ASCs promoted and inhibited differentiation, respectively. The pro-adipogenic activity of EST in humans was opposite to the anti-adipogenic effect of the same enzyme in rodents. Mechanistically, EST promoted adipogenesis by deactivating estrogens. The pro-adipogenic effect of EST can be recapitulated by using an estrogen receptor (ER) antagonist or ERα knockdown. In contrast, activation of ER in ASCs inhibited adipogenesis by decreasing the recruitment of the adipogenic peroxisome proliferator activated receptor γ (PPARγ) onto its target gene promoters, whereas ER antagonism increased the recruitment of PPARγ to its target gene promoters. Linear regression analysis revealed a positive correlation between the expression of EST and body mass index (BMI), as well as a negative correlation between the ERα expression and BMI. We conclude that EST is a pro-adipogenic factor, which may serve as a druggable target to inhibit the turnover and accumulation of adipocytes in obese patients. |
| Links |
PubMed Online version:10.1128/MCB.01147-13 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0045600: positive regulation of fat cell differentiation |
ECO:0000315: |
P |
Figure 2A and 2C show that preadipocytes in which SULT1E1 mRNA was overexpressed had higher rates of adipogenesis than the control preadipocytes with low levels of SULT1E1. |
complete | ||||
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involved_in |
GO:0045600: positive regulation of fat cell differentiation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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