GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:MichSt14A 33

From GONUTS
Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptablePYRFU:CAS6Findley4, MichSt14A 332014-04-06 14:45:52 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:19141480ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that cas6 cleaves RNA in repeat sequences of CRISPR elements.

challenge
unacceptableHALVD:D4GQN4Findley4, MichSt14A 332014-04-06 14:00:04 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:24459147ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows that a cas6 deletion mutant cannot generate crRNA.

challenge
updatedbyinstructorHALVD:CAS5Findley4, MichSt14A 332014-04-06 13:57:24 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:24459147ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows by complementation tests that a complex of cas5, cas6, and cas7 are necessary for crRNA processing

challenge
acceptableBACHD:Q9KFY3Findley4, MichSt14A 332014-04-06 13:06:08 CDTGO:0004518 nuclease activity (F)PMID:24371266ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that cas5d (BH0337 protein) has DNase activity on linear dsDNA, circular dsDNA, and ssDNA.

challenge
acceptableBACHD:Q9KFY3Findley4, MichSt14A 332014-04-06 12:51:03 CDTGO:0004521 endoribonuclease activity (F)PMID:22841292ECO:0000314 direct assay evidence used in manual assertion

Figure 1D shows that cas5d (BH0337 protein) has endoribonuclease activity because it can cleave RNA.

challenge
acceptableBACHD:Q9KFY3Findley4, MichSt14A 332014-04-06 12:41:50 CDTGO:0043571 maintenance of CRISPR repeat elements (P)PMID:22841292ECO:0000314 direct assay evidence used in manual assertion

Figure 1C shows that Cas5d (BH0337 protein) processes pre-crRNA in vitro.

challenge
acceptableHUMAN:ST1E1Findley4, MichSt14A 332014-04-06 11:50:26 CDTGO:0045600 positive regulation of fat cell differentiation (P)PMID:24567372ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A and 2C show that preadipocytes in which SULT1E1 mRNA was overexpressed had higher rates of adipogenesis than the control preadipocytes with low levels of SULT1E1.

challenge

What do the icons mean in the status column?

assessment icons.jpg

Pages in category "MichSt14A 33"

This category contains only the following page.