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User:Charlatd

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableVIBC3:E4PD2012-04-15 09:17:34 CDTGO:0048001 erythrose-4-phosphate dehydrogenase activity (F)PMID:8982014ECO:0000314 direct assay evidence used in manual assertion

Table 2: enzymatic activity of erythrose-4-phosphate dehydrogenase (Epd or E4PDH) in Vibrio cholerae. The set up of the assay is indicated beginning on page 293 on the last paragraph.

Plasmids were constructed containing epd and activity of each was measured by the amount of NADH production over 60 seconds.

An immediate downstream gene of epd is igrA, an iron-regulated outer membrane virulence gene. The results in table 2 indicate that in iron-deficient conditions, the activity of epd is not effected, whereas igrA is affected based on other published studies.

challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Charlatd

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptablePaxhiami,
Team Awesome Panthers
ECOLI:RFACGO:0008920 - lipopolysaccharide heptosyltransferase activity (F)PMID:11054112ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
acceptablePaxhiami,
Team Awesome Panthers
ECOLI:RFAFGO:0008713 - ADP-heptose-lipopolysaccharide heptosyltransferase activity (F)PMID:11054112ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Charlatd


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks

acceptable:0
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Charlatd

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptablePaxhiami,
Team Awesome Panthers
ECOLI:RFACGO:0008920 - lipopolysaccharide heptosyltransferase activity (F)PMID:11054112ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
acceptablePaxhiami,
Team Awesome Panthers
ECOLI:RFAFGO:0008713 - ADP-heptose-lipopolysaccharide heptosyltransferase activity (F)PMID:11054112ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

0 annotations fixed by Charlatd