GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:8982014
| Citation |
Carroll, PA, Zhao, G, Boyko, SA, Winkler, ME and Calderwood, SB (1997) Identification, sequencing, and enzymatic activity of the erythrose-4-phosphate dehydrogenase gene of Vibrio cholerae. J. Bacteriol. 179:293-6 |
|---|---|
| Abstract |
We have identified a gene in Vibrio cholerae (epd) which encodes an erythrose-4-phosphate dehydrogenase activity and is located immediately downstream of an iron-regulated virulence gene, irgA, and immediately upstream of a gene encoding phosphoglycerate kinase (pgk). Expression of epd in V. cholerae is not regulated by iron, nor is it required for virulence in an infant mouse model. |
| Links | |
| Keywords |
Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial/genetics; Glyceraldehyde-3-Phosphate Dehydrogenases/genetics; Iron/physiology; Molecular Sequence Data; Phosphoglycerate Kinase/genetics; Receptors, Cell Surface; Restriction Mapping; Sequence Homology, Amino Acid; Vibrio cholerae/enzymology; Vibrio cholerae/genetics; Vibrio cholerae/pathogenicity; Virulence |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0048001: erythrose-4-phosphate dehydrogenase activity |
ECO:0000314: |
F |
Table 2: enzymatic activity of erythrose-4-phosphate dehydrogenase (Epd or E4PDH) in Vibrio cholerae. The set up of the assay is indicated beginning on page 293 on the last paragraph. Plasmids were constructed containing epd and activity of each was measured by the amount of NADH production over 60 seconds. An immediate downstream gene of epd is igrA, an iron-regulated outer membrane virulence gene. The results in table 2 indicate that in iron-deficient conditions, the activity of epd is not effected, whereas igrA is affected based on other published studies. |
complete | ||||
|
enables |
GO:0048001: erythrose-4-phosphate dehydrogenase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
