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User:Bozekjen

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesPSESP:Q523872012-04-12 11:34:18 CDTGO:0018501 cis-chlorobenzene dihydrodiol dehydrogenase activity (F)PMID:1987135ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows that Pseudomonas sp. strain P51 is able to grow on culture medium that contained 3 different chlorobenzenes, and it was able to degrade the chlorobenzenes. Figure 7 shows how the chlorobenzene concentrations decreased, and the corresponding catechol concentrations increased.

challenge
requireschangesECOLI:PRPC2012-04-12 12:03:52 CDTGO:0050440 2-methylcitrate synthase activity (F)PMID:9579066ECO:0000314 direct assay evidence used in manual assertion

Table 1 shows a significant increase in 2-methylcitrate synthase activity when the carbon source in the growth medium was propionate.

challenge
acceptableYEAST:KICH2012-04-14 13:33:00 CDTGO:0004103 choline kinase activity (F)PMID:2536698ECO:0000315 mutant phenotype evidence used in manual assertion

Table 4 shows the close relationship between choline kinase activity and ethanolamine kinase activity. The wild type had both choline kinase activity and ethanolamine kinase activity. pCK1D overproduced choline kinase, and it also enhanced the ethanolamine kinase activity of the transformant. pCK1G failed to restore the choline kinase activity or the ethanolamine kinase activity.

challenge
acceptableCLOAB:ADHE2012-04-15 10:46:56 CDTGO:0004022 alcohol dehydrogenase (NAD) activity (F)PMID:18726959ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows that overexpression of the alcohol/aldehyde dehydrogenase gene increased the production of butanol and ethanol.

challenge
acceptableKLEPN:AGLB2012-04-15 12:17:44 CDTGO:0050081 maltose-6'-phosphate glucosidase activity (F)PMID:11473129ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows the sugar-specific induction of aglB, 6'-phospho-alpha-glucosidase. It was active in all the 6'-phosphorylated isomers of glucose, but not in sucrose.

challenge
acceptableECOLI:ACSA2012-04-15 13:39:01 CDTGO:0003987 acetate-CoA ligase activity (F)PMID:7751300ECO:0000314 direct assay evidence used in manual assertion

Figure 9. Also, Table 3 shows that Acs deficient strains were not able to activate acetate as well as the wild-type strain.

challenge

acceptable:4
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Bozekjen

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Bozekjen


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:CYNS2012-03-29 12:14:37 CDTGO:0008824 cyanate hydratase activity (F)PMID:3309165ECO:0000315 mutant phenotype evidence used in manual assertion

Table 9 shows that wild-type E. coli strains were able to grow on medium containing 0.5 M urea and mutants lacking cyanase were not.

challenge
requireschangesARTAT:A1R5Y52012-03-30 20:56:46 CDTGO:0018814 phenylacetaldoxime dehydratase activity (F)PMID:12773527ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows that OxdA activity is inducible when butyraldoxime was used in low concentrations as the sole carbon and nitrogen source. No OxdA activity was observed when sucrose and (NH4)2SO4 were used as the carbon and nitrogen sources.

challenge

acceptable:0
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Bozekjen

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Bozekjen