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SCHPO:PIF1

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Species (Taxon ID) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812)
Gene Name(s) pfh1 (synonyms: pif1, rph1)
Protein Name(s) ATP-dependent DNA helicase pfh1

DNA repair and recombination helicase pfh1 PIF1 helicase homolog RRM3/PIF1 homolog 1

External Links
UniProt Q9UUA2
EMBL AF074944
CU329671
PIR T40739
T47241
RefSeq NP_596488.1
BioGrid 277727
MINT MINT-4711480
iPTMnet Q9UUA2
MaxQB Q9UUA2
EnsemblFungi SPBC887.14c.1
GeneID 2541213
KEGG spo:SPBC887.14c
EuPathDB FungiDB:SPBC887.14c
PomBase SPBC887.14c
HOGENOM HOG000132960
InParanoid Q9UUA2
KO K15255
OrthoDB EOG76MKKK
PhylomeDB Q9UUA2
BRENDA 3.6.4.12
PRO PR:Q9UUA2
Proteomes UP000002485
GO GO:0005739
GO:0000790
GO:0005730
GO:0005634
GO:0035861
GO:0005524
GO:0043141
GO:0016887
GO:0003677
GO:0017116
GO:0032508
GO:0006310
GO:1902983
GO:0044806
GO:0043504
GO:1903469
GO:0000723
Gene3D 3.40.50.300
HAMAP MF_03176
InterPro IPR003593
IPR003840
IPR010285
IPR027417
PANTHER PTHR23274
Pfam PF02689
PF05970
SMART SM00382
SUPFAM SSF52540

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000002

mitochondrial genome maintenance

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

P

Seeded From UniProt

GO:0000166

nucleotide binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0547

F

Seeded From UniProt

GO:0000723

telomere maintenance

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010285

P

Seeded From UniProt

GO:0000790

nuclear chromatin

PMID:22426534[1]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0000790

nuclear chromatin

PMID:22426534[1]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0000790

nuclear chromatin

PMID:22426534[1]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0000790

nuclear chromatin

PMID:22426534[1]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0000790

nuclear chromatin

PMID:22426534[1]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0003677

DNA binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0238

F

Seeded From UniProt

GO:0003678

DNA helicase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010285

F

Seeded From UniProt

GO:0004386

helicase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003840

F

Seeded From UniProt

GO:0004386

helicase activity

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0347

F

Seeded From UniProt

GO:0005524

ATP binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003840

F

Seeded From UniProt

GO:0005524

ATP binding

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

F

Seeded From UniProt

GO:0005524

ATP binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0067

F

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

C

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

Seeded From UniProt

GO:0005634

nucleus

PMID:12409464[2]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005634

nucleus

PMID:16823372[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005634

nucleus

PMID:18725402[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005657

replication fork

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN001149889

C

Seeded From UniProt

GO:0005730

nucleolus

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0188

C

Seeded From UniProt

GO:0005730

nucleolus

PMID:18725402[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005739

mitochondrion

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

C

Seeded From UniProt

GO:0005739

mitochondrion

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0496

C

Seeded From UniProt

GO:0005739

mitochondrion

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0173

C

Seeded From UniProt

GO:0005739

mitochondrion

PMID:16823372[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005739

mitochondrion

PMID:18725402[4]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0006281

DNA repair

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010285

P

Seeded From UniProt

GO:0006281

DNA repair

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

P

Seeded From UniProt

GO:0006281

DNA repair

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0234

P

Seeded From UniProt

GO:0006310

DNA recombination

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

P

Seeded From UniProt

GO:0006310

DNA recombination

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0233

P

Seeded From UniProt

GO:0006974

cellular response to DNA damage stimulus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0227

P

Seeded From UniProt

GO:0010521

telomerase inhibitor activity

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN001512181

F

Seeded From UniProt

GO:0016787

hydrolase activity

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0378

F

Seeded From UniProt

GO:0016887

ATPase activity

PMID:15302919[5]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0017116

single-stranded DNA-dependent ATP-dependent DNA helicase activity

PMID:15302919[5]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0032211

negative regulation of telomere maintenance via telomerase

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN001512181

P

Seeded From UniProt

GO:0032508

DNA duplex unwinding

PMID:12058079[6]

IDA: Inferred from Direct Assay

-

P

Seeded From UniProt

GO:0035861

site of double-strand break

PMID:23628481[7]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_03176

F

Seeded From UniProt

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:12058079[6]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:12409464[2]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:15302919[5]

IDA: Inferred from Direct Assay

-

F

Seeded From UniProt

GO:0043504

mitochondrial DNA repair

PMID:18725402[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0044806

G-quadruplex DNA unwinding

PMID:26041456[8]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0051974

negative regulation of telomerase activity

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN001512181

P

Seeded From UniProt

GO:1902983

DNA strand elongation involved in mitotic DNA replication

PMID:22426534[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:1902983

DNA strand elongation involved in mitotic DNA replication

PMID:22426534[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:1902983

DNA strand elongation involved in mitotic DNA replication

PMID:22426534[1]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:1903469

removal of RNA primer involved in mitotic DNA replication

PMID:12409464[2]

IGI: Inferred from Genetic Interaction

PomBase:SPAC8F11.07c

P

Seeded From UniProt

GO:1903469

removal of RNA primer involved in mitotic DNA replication

PMID:15302919[5]

IGI: Inferred from Genetic Interaction

PomBase:SPBC16D10.04c

P

Seeded From UniProt

GO:0033128

negative regulation of histone phosphorylation

PMID:27611590[9]

IMP: Inferred from Mutant Phenotype

P

Figure 3, parts B-F, show that in the absence of pfh-1 protein there was more phosphorylated histone protein indicating increased DNA damage.

complete
CACAO 12101

GO:0006974

cellular response to DNA damage stimulus

PMID:27611590[9]

IMP: Inferred from Mutant Phenotype

P

Figure 3, parts B-F, show that in the absence of pfh-1 protein there was more phosphorylated histone protein indicating increased DNA damage.

complete
CACAO 12102

GO:0031297

replication fork processing

PMID:27611590[9]

IMP: Inferred from Mutant Phenotype

P

In figure 2, they used Cdc 20 occupancy as a way to denote pausing within the replication fork. They found that in cells where Pfh1 was absent, Cdc 20 occupancy was increased.

complete
CACAO 12121

GO:0031297

replication fork processing

PMID:22426535[10]

IMP: Inferred from Mutant Phenotype

P

Fig. 2 B-D states how Pfh1 promotes replication fork progression at a tRNA gene, with it performing a sweepase function in promoting replication fork passage. A child term was created for this regarding progress and tRNA genes. The organism is S. pombe.

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Sabouri, N et al. (2012) DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase. Genes Dev. 26 581-93 PubMed GONUTS page
  2. 2.0 2.1 2.2 Tanaka, H et al. (2002) The fission yeast pfh1(+) gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase. Nucleic Acids Res. 30 4728-39 PubMed GONUTS page
  3. 3.0 3.1 Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
  4. 4.0 4.1 4.2 4.3 Pinter, SF et al. (2008) The Schizosaccharomyces pombe Pfh1p DNA helicase is essential for the maintenance of nuclear and mitochondrial DNA. Mol. Cell. Biol. 28 6594-608 PubMed GONUTS page
  5. 5.0 5.1 5.2 5.3 Ryu, GH et al. (2004) Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast. Nucleic Acids Res. 32 4205-16 PubMed GONUTS page
  6. 6.0 6.1 Zhou, JQ et al. (2002) Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases. Mol. Biol. Cell 13 2180-91 PubMed GONUTS page
  7. Yu, Y et al. (2013) A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks. DNA Repair (Amst.) 12 433-43 PubMed GONUTS page
  8. Audry, J et al. (2015) RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends. EMBO J. 34 1942-58 PubMed GONUTS page
  9. 9.0 9.1 9.2 McDonald, KR et al. (2016) Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity. PLoS Genet. 12 e1006238 PubMed GONUTS page
  10. Steinacher, R et al. (2012) The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability. Genes Dev. 26 594-602 PubMed GONUTS page