GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

SCHPO:PIF1

From GONUTS
Jump to: navigation, search
Species (Taxon ID) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812)
Gene Name(s) pfh1 (synonyms: pif1, rph1)
Protein Name(s) ATP-dependent DNA helicase pfh1

DNA repair and recombination helicase pfh1 PIF1 helicase homolog RRM3/PIF1 homolog 1

External Links
UniProt Q9UUA2
EMBL AF074944
CU329671
PIR T40739
T47241
RefSeq NP_596488.1
ProteinModelPortal Q9UUA2
BioGrid 277727
STRING 4896.SPBC887.14c.1
iPTMnet Q9UUA2
PaxDb Q9UUA2
PRIDE Q9UUA2
EnsemblFungi SPBC887.14c.1
GeneID 2541213
KEGG spo:SPBC887.14c
EuPathDB FungiDB:SPBC887.14c
PomBase SPBC887.14c
HOGENOM HOG000132960
InParanoid Q9UUA2
KO K15255
OMA QRETWNI
PhylomeDB Q9UUA2
BRENDA 3.6.4.12
PRO PR:Q9UUA2
Proteomes UP000002485
GO GO:0005739
GO:0000790
GO:1902377
GO:0043601
GO:0005724
GO:0005730
GO:0005634
GO:0005657
GO:0035861
GO:0005524
GO:0043141
GO:0033682
GO:0061995
GO:0016887
GO:1990814
GO:0070336
GO:0051880
GO:0003723
GO:1990518
GO:0017116
GO:0032508
GO:0006310
GO:0006260
GO:1902983
GO:0044806
GO:0043504
GO:1905467
GO:1903469
GO:0031297
GO:0000723
HAMAP MF_03176
InterPro IPR003593
IPR003840
IPR010285
IPR027417
Pfam PF02689
PF05970
SMART SM00382
SUPFAM SSF52540

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0033128

negative regulation of histone phosphorylation

PMID:27611590[1]

ECO:0000315

P

Figure 3, parts B-F, show that in the absence of pfh-1 protein there was more phosphorylated histone protein indicating increased DNA damage.

complete
CACAO 12101

GO:0006974

cellular response to DNA damage stimulus

PMID:27611590[1]

ECO:0000315

P

Figure 3, parts B-F, show that in the absence of pfh-1 protein there was more phosphorylated histone protein indicating increased DNA damage.

complete
CACAO 12102

GO:0031297

replication fork processing

PMID:27611590[1]

ECO:0000315

P

In figure 2, they used Cdc 20 occupancy as a way to denote pausing within the replication fork. They found that in cells where Pfh1 was absent, Cdc 20 occupancy was increased.

complete
CACAO 12121

GO:0031297

replication fork processing

PMID:22426535[2]

ECO:0000315

P

Fig. 2 B-D states how Pfh1 promotes replication fork progression at a tRNA gene, with it performing a sweepase function in promoting replication fork passage. A child term was created for this regarding progress and tRNA genes. The organism is S. pombe.

complete
CACAO 12234

part_of

GO:0035861

site of double-strand break

PMID:23628481[3]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0033682

ATP-dependent 5'-3' DNA/RNA helicase activity

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0032508

DNA duplex unwinding

PMID:12058079[5]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0031297

replication fork processing

PMID:27611590[1]

ECO:0000269

experimental evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0017116

single-stranded DNA-dependent ATP-dependent DNA helicase activity

PMID:15302919[6]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0016887

ATPase activity

PMID:15302919[6]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:0006310

DNA recombination

GO_REF:0000024

ECO:0000266

sequence orthology evidence used in manual assertion

SGD:S000004526

P

Seeded From UniProt

complete

part_of

GO:0005739

mitochondrion

PMID:16823372[7]

ECO:0007005

high throughput direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005739

mitochondrion

PMID:18725402[8]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005730

nucleolus

PMID:18725402[8]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005724

nuclear telomeric heterochromatin

PMID:27185885[9]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0000003)

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:16823372[7]

ECO:0007005

high throughput direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:18725402[8]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

PMID:12409464[10]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000050

ECO:0000255

match to sequence model evidence used in manual assertion

InterPro:IPR003593

F

Seeded From UniProt

complete

enables

GO:0003723

RNA binding

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:27611590[1]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0001637)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:27611590[1]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0001272)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:27611590[1]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0000339)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:27611590[1]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0000205)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:25471935[11]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0001637)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:25471935[11]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0000003)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:22426534[12]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0002021)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:22426534[12]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0001914)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:22426534[12]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(PomBase:SPCC622.09)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:22426534[12]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(PomBase:SPCC622.08c)

Seeded From UniProt

complete

part_of

GO:0000790

nuclear chromatin

PMID:22426534[12]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(PomBase:SPBC32H8.12c)

Seeded From UniProt

complete

part_of

GO:0000262

mitochondrial chromosome

GO_REF:0000111

ECO:0000305

curator inference used in manual assertion

GO:0003677
GO:0005739

C

Seeded From UniProt

complete

enables

GO:1990814

DNA/DNA annealing activity

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:1990518

single-stranded DNA-dependent ATP-dependent 3'-5' DNA helicase activity

PMID:15302919[6]

ECO:0000305

curator inference used in manual assertion

GO:0017116
GO:0043141

F

Seeded From UniProt

complete

involved_in

GO:1905467

positive regulation of G-quadruplex DNA unwinding

PMID:25471935[11]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:1903469

removal of RNA primer involved in mitotic DNA replication

PMID:15302919[6]

ECO:0000316

genetic interaction evidence used in manual assertion

PomBase:SPBC16D10.04c

P

Seeded From UniProt

complete

involved_in

GO:1903469

removal of RNA primer involved in mitotic DNA replication

PMID:12409464[10]

ECO:0000316

genetic interaction evidence used in manual assertion

PomBase:SPAC8F11.07c

P

Seeded From UniProt

complete

involved_in

GO:1902983

DNA strand elongation involved in mitotic DNA replication

PMID:22426534[12]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

occurs_at:(SO:0002021)

Seeded From UniProt

complete

involved_in

GO:1902983

DNA strand elongation involved in mitotic DNA replication

PMID:22426534[12]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

occurs_at:(SO:0001914)

Seeded From UniProt

complete

involved_in

GO:1902983

DNA strand elongation involved in mitotic DNA replication

PMID:22426534[12]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

occurs_at:(SO:0001272)

Seeded From UniProt

complete

part_of

GO:1902377

nuclear rDNA heterochromatin

PMID:27185885[9]

ECO:0000314

direct assay evidence used in manual assertion

C

coincident_with:(SO:0000003)

Seeded From UniProt

complete

enables

GO:0070336

flap-structured DNA binding

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0061995

ATP-dependent protein-DNA complex displacement activity

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0051880

G-quadruplex DNA binding

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0051880

G-quadruplex DNA binding

PMID:27185885[9]

ECO:0000314

direct assay evidence used in manual assertion

F

occurs_at:(SO:0001637)

Seeded From UniProt

complete

enables

GO:0051880

G-quadruplex DNA binding

PMID:27185885[9]

ECO:0000314

direct assay evidence used in manual assertion

F

occurs_at:(SO:0000624)

Seeded From UniProt

complete

involved_in

GO:0044806

G-quadruplex DNA unwinding

PMID:26041456[13]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0044806

G-quadruplex DNA unwinding

PMID:30053106[4]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0044806

G-quadruplex DNA unwinding

PMID:27185885[9]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0043601

nuclear replisome

PMID:27611590[1]

ECO:0000269

experimental evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0043504

mitochondrial DNA repair

PMID:18725402[8]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:27185885[9]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:15302919[6]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:12409464[10]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:12058079[5]

ECO:0000314

direct assay evidence used in manual assertion

F

activated_by:(CHEBI:18420)

Seeded From UniProt

complete

enables

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN001149889
PomBase:SPBC887.14c
UniProtKB:Q9H611

F

Seeded From UniProt

complete

involved_in

GO:0032508

DNA duplex unwinding

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN001149889
PomBase:SPBC887.14c

P

Seeded From UniProt

complete

involved_in

GO:0006260

DNA replication

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN001149889
SGD:S000001073

P

Seeded From UniProt

complete

part_of

GO:0005657

replication fork

PMID:21873635[14]

ECO:0000318

biological aspect of ancestor evidence used in manual assertion

PANTHER:PTN001149889
SGD:S000001073
SGD:S000004526

C

Seeded From UniProt

complete

involved_in

GO:0000723

telomere maintenance

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR010285

P

Seeded From UniProt

complete

enables

GO:0003678

DNA helicase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR010285

F

Seeded From UniProt

complete

enables

GO:0004386

helicase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003840

F

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR003840

F

Seeded From UniProt

complete

involved_in

GO:0006281

DNA repair

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR010285

P

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

F

Seeded From UniProt

complete

involved_in

GO:0006281

DNA repair

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

P

Seeded From UniProt

complete

enables

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

F

Seeded From UniProt

complete

involved_in

GO:0000002

mitochondrial genome maintenance

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

P

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

C

Seeded From UniProt

complete

part_of

GO:0005739

mitochondrion

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

C

Seeded From UniProt

complete

involved_in

GO:0006310

DNA recombination

GO_REF:0000104

ECO:0000256

match to sequence model evidence used in automatic assertion

UniRule:UR000112749

P

Seeded From UniProt

complete

involved_in

GO:0006974

cellular response to DNA damage stimulus

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0227

P

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

enables

GO:0004386

helicase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0347

F

Seeded From UniProt

complete

part_of

GO:0005739

mitochondrion

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0496
UniProtKB-SubCell:SL-0173

C

Seeded From UniProt

complete

involved_in

GO:0006281

DNA repair

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0234

P

Seeded From UniProt

complete

involved_in

GO:0006310

DNA recombination

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0233

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

enables

GO:0005524

ATP binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0067

F

Seeded From UniProt

complete

part_of

GO:0005634

nucleus

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0539

C

Seeded From UniProt

complete

enables

GO:0000166

nucleotide binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

part_of

GO:0005730

nucleolus

GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-SubCell:SL-0188

C

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 McDonald, KR et al. (2016) Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity. PLoS Genet. 12 e1006238 PubMed GONUTS page
  2. Steinacher, R et al. (2012) The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability. Genes Dev. 26 594-602 PubMed GONUTS page
  3. Yu, Y et al. (2013) A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks. DNA Repair (Amst.) 12 433-43 PubMed GONUTS page
  4. 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Mohammad, JB et al. (2018) The Pif1 signature motif of Pfh1 is necessary for both protein displacement and helicase unwinding activities, but is dispensable for strand-annealing activity. Nucleic Acids Res. PubMed GONUTS page
  5. 5.0 5.1 Zhou, JQ et al. (2002) Schizosaccharomyces pombe pfh1+ encodes an essential 5' to 3' DNA helicase that is a member of the PIF1 subfamily of DNA helicases. Mol. Biol. Cell 13 2180-91 PubMed GONUTS page
  6. 6.0 6.1 6.2 6.3 6.4 Ryu, GH et al. (2004) Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast. Nucleic Acids Res. 32 4205-16 PubMed GONUTS page
  7. 7.0 7.1 Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
  8. 8.0 8.1 8.2 8.3 Pinter, SF et al. (2008) The Schizosaccharomyces pombe Pfh1p DNA helicase is essential for the maintenance of nuclear and mitochondrial DNA. Mol. Cell. Biol. 28 6594-608 PubMed GONUTS page
  9. 9.0 9.1 9.2 9.3 9.4 9.5 Wallgren, M et al. (2016) G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase. Nucleic Acids Res. 44 6213-31 PubMed GONUTS page
  10. 10.0 10.1 10.2 Tanaka, H et al. (2002) The fission yeast pfh1(+) gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase. Nucleic Acids Res. 30 4728-39 PubMed GONUTS page
  11. 11.0 11.1 11.2 Sabouri, N et al. (2014) The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage. BMC Biol. 12 101 PubMed GONUTS page
  12. 12.0 12.1 12.2 12.3 12.4 12.5 12.6 12.7 Sabouri, N et al. (2012) DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase. Genes Dev. 26 581-93 PubMed GONUTS page
  13. Audry, J et al. (2015) RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends. EMBO J. 34 1942-58 PubMed GONUTS page
  14. 14.0 14.1 14.2 14.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page