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PMID:22426535
| Citation |
Steinacher, R, Osman, F, Dalgaard, JZ, Lorenz, A and Whitby, MC (2012) The DNA helicase Pfh1 promotes fork merging at replication termination sites to ensure genome stability. Genes Dev. 26:594-602 |
|---|---|
| Abstract |
Bidirectionally moving DNA replication forks merge at termination sites composed of accidental or programmed DNA-protein barriers. If merging fails, then regions of unreplicated DNA can result in the breakage of DNA during mitosis, which in turn can give rise to genome instability. Despite its importance, little is known about the mechanisms that promote the final stages of fork merging in eukaryotes. Here we show that the Pif1 family DNA helicase Pfh1 plays a dual role in promoting replication fork termination. First, it facilitates replication past DNA-protein barriers, and second, it promotes the merging of replication forks. A failure of these processes in Pfh1-deficient cells results in aberrant chromosome segregation and heightened genome instability. |
| Links |
PubMed PMC3315120 Online version:10.1101/gad.184663.111 |
| Keywords |
Chromosome Segregation; DNA Helicases/genetics; DNA Helicases/metabolism; DNA Replication; DNA, Fungal/genetics; DNA, Ribosomal/genetics; Genomic Instability; Schizosaccharomyces/enzymology; Schizosaccharomyces/genetics; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0031297: replication fork processing |
ECO:0000315: |
P |
Fig. 2 B-D states how Pfh1 promotes replication fork progression at a tRNA gene, with it performing a sweepase function in promoting replication fork passage. A child term was created for this regarding progress and tRNA genes. The organism is S. pombe. |
complete | ||||
Notes
See also
References
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