PMID:18516287

Citation	Berger, J, Senti, KA, Senti, G, Newsome, TP, Asling, B, Dickson, BJ and Suzuki, T (2008) Systematic identification of genes that regulate neuronal wiring in the Drosophila visual system. PLoS Genet. 4:e1000085
Abstract	Forward genetic screens in model organisms are an attractive means to identify those genes involved in any complex biological process, including neural circuit assembly. Although mutagenesis screens are readily performed to saturation, gene identification rarely is, being limited by the considerable effort generally required for positional cloning. Here, we apply a systematic positional cloning strategy to identify many of the genes required for neuronal wiring in the Drosophila visual system. From a large-scale forward genetic screen selecting for visual system wiring defects with a normal retinal pattern, we recovered 122 mutations in 42 genetic loci. For 6 of these loci, the underlying genetic lesions were previously identified using traditional methods. Using SNP-based mapping approaches, we have now identified 30 additional genes. Neuronal phenotypes have not previously been reported for 20 of these genes, and no mutant phenotype has been previously described for 5 genes. The genes encode a variety of proteins implicated in cellular processes such as gene regulation, cytoskeletal dynamics, axonal transport, and cell signalling. We conducted a comprehensive phenotypic analysis of 35 genes, scoring wiring defects according to 33 criteria. This work demonstrates the feasibility of combining large-scale gene identification with large-scale mutagenesis in Drosophila, and provides a comprehensive overview of the molecular mechanisms that regulate visual system wiring.
Links	PubMed PMC2377342 Online version:10.1371/journal.pgen.1000085
Keywords	Animals; Axons/physiology; Cloning, Molecular; Drosophila/genetics; Drosophila/physiology; Drosophila Proteins/genetics; Mutation; Nervous System/metabolism; Phenotype; Polymorphism, Single Nucleotide; Retina/metabolism
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:LAR	involved_in	GO:0007411: axon guidance	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:JNK	involved_in	GO:0007411: axon guidance	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:PROF	involved_in	GO:0007411: axon guidance	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9Y1L3	involved_in	GO:0007411: axon guidance	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:PTP69	involved_in	GO:0007411: axon guidance	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:RB27C	involved_in	GO:0007411: axon guidance	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.