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DROME:POLO

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Species (Taxon ID) Drosophila melanogaster (Fruit fly). (7227)
Gene Name(s) polo
Protein Name(s) Serine/threonine-protein kinase polo
External Links
UniProt P52304
EMBL X63361
AE014296
AE014296
AY095028
PIR S22127
RefSeq NP_001014592.1
NP_524179.2
UniGene Dm.2513
ProteinModelPortal P52304
SMR P52304
BioGrid 65494
DIP DIP-18462N
IntAct P52304
MINT MINT-1562298
PaxDb P52304
EnsemblMetazoa FBtr0074839
FBtr0100318
GeneID 40232
KEGG dme:Dmel_CG12306
CTD 40232
FlyBase FBgn0003124
eggNOG COG0515
GeneTree ENSGT00530000062954
InParanoid P52304
KO K06631
OMA YQKIRYA
OrthoDB EOG78M01K
PhylomeDB P52304
BRENDA 2.7.11.21
Reactome REACT_184294
REACT_206225
REACT_208929
REACT_231708
REACT_233284
REACT_238855
REACT_245412
REACT_251099
SignaLink P52304
ChiTaRS polo
GenomeRNAi 40232
NextBio 817698
PRO PR:P52304
Proteomes UP000000803
Bgee P52304
ExpressionAtlas P52304
GO GO:0005814
GO:0005813
GO:0000940
GO:0005737
GO:0000776
GO:0005828
GO:0005874
GO:0030496
GO:1990023
GO:0005635
GO:0005819
GO:0000922
GO:0005524
GO:0004672
GO:0004674
GO:0000915
GO:0030954
GO:0034613
GO:0051297
GO:0035050
GO:0007147
GO:0007143
GO:0030726
GO:0007140
GO:0007060
GO:0007112
GO:0051257
GO:0000278
GO:0007067
GO:0007052
GO:0007406
GO:0022008
GO:0048600
GO:0006909
GO:0007344
GO:0035046
GO:0008104
GO:0006468
GO:0035044
GO:0007058
InterPro IPR011009
IPR000959
IPR000719
IPR002290
IPR008271
Pfam PF00069
PF00659
SMART SM00220
SUPFAM SSF56112
PROSITE PS50078
PS50011
PS00108

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0051315

attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation

PMID:22389397[1]

IDA: Inferred from Direct Assay

P

Figure 1 shows that POLO depletion leads to chromosomes attaching to the mitotic spindle with syntelic orientation.

complete
CACAO 6645

GO:0000166

nucleotide binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0547

F

Seeded From UniProt

complete

GO:0000278

mitotic cell cycle

PMID:14527345[2]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0000278

mitotic cell cycle

PMID:18832073[3]

IGI: Inferred from Genetic Interaction

FB:FBgn0002645

P

Seeded From UniProt

complete

GO:0000278

mitotic cell cycle

PMID:18832073[3]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0000776

kinetochore

PMID:15572022[4]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0000776

kinetochore

PMID:17046997[5]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0000776

kinetochore

PMID:18798690[6]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0000776

kinetochore

PMID:18832073[3]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0000915

actomyosin contractile ring assembly

PMID:9813088[7]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0000922

spindle pole

PMID:15572022[4]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0000940

condensed chromosome outer kinetochore

PMID:11483958[8]

NAS: Non-traceable Author Statement

C

Seeded From UniProt

complete

GO:0000940

condensed chromosome outer kinetochore

PMID:19529778[9]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0004672

protein kinase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000719
InterPro:IPR002290
InterPro:IPR008271

F

Seeded From UniProt

complete

GO:0004672

protein kinase activity

PMID:10908587[10]

NAS: Non-traceable Author Statement

F

Seeded From UniProt

complete

GO:0004672

protein kinase activity

PMID:11283617[11]

IDA: Inferred from Direct Assay

F

Seeded From UniProt

complete

GO:0004672

protein kinase activity

PMID:23857770[12]

IDA: Inferred from Direct Assay

F

Seeded From UniProt

complete

GO:0004672

protein kinase activity

PMID:8510757[13]

IDA: Inferred from Direct Assay

F

Seeded From UniProt

complete

GO:0004674

protein serine/threonine kinase activity

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0723

F

Seeded From UniProt

complete

GO:0004674

protein serine/threonine kinase activity

PMID:1660828[14]

ISS: Inferred from Sequence or Structural Similarity

F

Seeded From UniProt

Missing: with/from

GO:0004674

protein serine/threonine kinase activity

PMID:8890161[15]

IDA: Inferred from Direct Assay

F

Seeded From UniProt

complete

GO:0005515

protein binding

PMID:23857770[12]

IPI: Inferred from Physical Interaction

FB:FBgn0260399

F

Seeded From UniProt

complete

GO:0005524

ATP binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000719
InterPro:IPR002290

F

Seeded From UniProt

complete

GO:0005524

ATP binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0067

F

Seeded From UniProt

complete

GO:0005635

nuclear envelope

PMID:18832073[3]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005737

cytoplasm

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0963

C

Seeded From UniProt

complete

GO:0005737

cytoplasm

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

GO:0005737

cytoplasm

PMID:19529778[9]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005813

centrosome

PMID:11283617[11]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005813

centrosome

PMID:18832073[3]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005813

centrosome

PMID:9914376[16]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005814

centriole

PMID:18798690[6]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005814

centriole

PMID:24954048[17]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005819

spindle

PMID:16412419[18]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005819

spindle

PMID:18832073[3]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005819

spindle

PMID:9914376[16]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005828

kinetochore microtubule

PMID:17046997[5]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0005874

microtubule

PMID:18832073[3]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0006468

protein phosphorylation

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000719
InterPro:IPR002290
InterPro:IPR008271

P

Seeded From UniProt

complete

GO:0006468

protein phosphorylation

PMID:10908587[10]

NAS: Non-traceable Author Statement

P

Seeded From UniProt

complete

GO:0006468

protein phosphorylation

PMID:11283617[11]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0006468

protein phosphorylation

PMID:23857770[12]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:0006909

phagocytosis

PMID:16336044[19]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007052

mitotic spindle organization

PMID:17412918[20]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007058

spindle assembly involved in female meiosis II

PMID:10954431[21]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007067

mitotic nuclear division

PMID:18463163[22]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007067

mitotic nuclear division

PMID:3417791[23]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007112

male meiosis cytokinesis

PMID:9813088[7]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007140

male meiosis

PMID:3417791[23]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007143

female meiotic division

PMID:16971474[24]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007147

female meiosis II

PMID:10954431[21]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007344

pronuclear fusion

PMID:10954431[21]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0007406

negative regulation of neuroblast proliferation

PMID:17805297[25]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0008104

protein localization

PMID:11258476[26]

NAS: Non-traceable Author Statement

P

Seeded From UniProt

complete

GO:0016301

kinase activity

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0418

F

Seeded From UniProt

complete

GO:0016310

phosphorylation

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0418

P

Seeded From UniProt

complete

GO:0016740

transferase activity

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

GO:0022008

neurogenesis

PMID:21549331[27]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0030496

midbody

PMID:16412419[18]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0030496

midbody

PMID:18798690[6]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0030496

midbody

PMID:19529778[9]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

GO:0030726

male germline ring canal formation

PMID:9813088[7]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0030954

astral microtubule nucleation

PMID:11283617[11]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0034613

cellular protein localization

PMID:9813088[7]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0035044

sperm aster formation

PMID:10954431[21]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0035046

pronuclear migration

PMID:10954431[21]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0035050

embryonic heart tube development

PMID:22814603[28]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0048600

oocyte fate commitment

PMID:23136393[29]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0051257

meiotic spindle midzone assembly

PMID:9813088[7]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0051297

centrosome organization

PMID:18798690[6]

IMP: Inferred from Mutant Phenotype

P

Seeded From UniProt

complete

GO:0051315

attachment of mitotic spindle microtubules to kinetochore

PMID:22389397[1]

IDA: Inferred from Direct Assay

P

Seeded From UniProt

complete

GO:1990023

mitotic spindle midzone

PMID:17046997[5]

IDA: Inferred from Direct Assay

C

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Moutinho-Santos, T et al. (2012) POLO ensures chromosome bi-orientation by preventing and correcting erroneous chromosome-spindle attachments. J. Cell. Sci. 125 576-83 PubMed GONUTS page
  2. Kiger, AA et al. (2003) A functional genomic analysis of cell morphology using RNA interference. J. Biol. 2 27 PubMed GONUTS page
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 Archambault, V et al. (2008) Sequestration of Polo kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by phosphorylation at a CDK site in mitosis. Genes Dev. 22 2707-20 PubMed GONUTS page
  4. 4.0 4.1 Prigent, C et al. (2005) Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure. Exp. Cell Res. 303 1-13 PubMed GONUTS page
  5. 5.0 5.1 5.2 Delcros, JG et al. (2006) Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells. J. Cell. Sci. 119 4431-41 PubMed GONUTS page
  6. 6.0 6.1 6.2 6.3 Dobbelaere, J et al. (2008) A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila. PLoS Biol. 6 e224 PubMed GONUTS page
  7. 7.0 7.1 7.2 7.3 7.4 Carmena, M et al. (1998) Drosophila polo kinase is required for cytokinesis. J. Cell Biol. 143 659-71 PubMed GONUTS page
  8. Blower, MD & Karpen, GH (2001) The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions. Nat. Cell Biol. 3 730-9 PubMed GONUTS page
  9. 9.0 9.1 9.2 Nie, M et al. (2009) Genetic and proteomic evidence for roles of Drosophila SUMO in cell cycle control, Ras signaling, and early pattern formation. PLoS ONE 4 e5905 PubMed GONUTS page
  10. 10.0 10.1 Morrison, DK et al. (2000) Protein kinases and phosphatases in the Drosophila genome. J. Cell Biol. 150 F57-62 PubMed GONUTS page
  11. 11.0 11.1 11.2 11.3 do Carmo Avides, M et al. (2001) Polo kinase and Asp are needed to promote the mitotic organizing activity of centrosomes. Nat. Cell Biol. 3 421-4 PubMed GONUTS page
  12. 12.0 12.1 12.2 Wang, P et al. (2013) Cell cycle regulation of Greatwall kinase nuclear localization facilitates mitotic progression. J. Cell Biol. 202 277-93 PubMed GONUTS page
  13. Fenton, B & Glover, DM (1993) A conserved mitotic kinase active at late anaphase-telophase in syncytial Drosophila embryos. Nature 363 637-40 PubMed GONUTS page
  14. Llamazares, S et al. (1991) polo encodes a protein kinase homolog required for mitosis in Drosophila. Genes Dev. 5 2153-65 PubMed GONUTS page
  15. Tavares, AA et al. (1996) The conserved mitotic kinase polo is regulated by phosphorylation and has preferred microtubule-associated substrates in Drosophila embryo extracts. EMBO J. 15 4873-83 PubMed GONUTS page
  16. 16.0 16.1 Herrmann, S et al. (1998) The POLO kinase is required at multiple stages during spermatogenesis in Drosophila melanogaster. Chromosoma 107 440-51 PubMed GONUTS page
  17. Singh, P et al. (2014) The centriolar protein Bld10/Cep135 is required to establish centrosome asymmetry in Drosophila neuroblasts. Curr. Biol. 24 1548-55 PubMed GONUTS page
  18. 18.0 18.1 Pearson, J et al. (2006) Heterologous expression of mammalian Plk1 in Drosophila reveals divergence from Polo during late mitosis. Exp. Cell Res. 312 770-81 PubMed GONUTS page
  19. Stroschein-Stevenson, SL et al. (2006) Identification of Drosophila gene products required for phagocytosis of Candida albicans. PLoS Biol. 4 e4 PubMed GONUTS page
  20. Goshima, G et al. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316 417-21 PubMed GONUTS page
  21. 21.0 21.1 21.2 21.3 21.4 Riparbelli, MG et al. (2000) Failure of pronuclear migration and repeated divisions of polar body nuclei associated with MTOC defects in polo eggs of Drosophila. J. Cell. Sci. 113 ( Pt 18) 3341-50 PubMed GONUTS page
  22. Ducat, D et al. (2008) Regulation of microtubule assembly and organization in mitosis by the AAA+ ATPase Pontin. Mol. Biol. Cell 19 3097-110 PubMed GONUTS page
  23. 23.0 23.1 Sunkel, CE & Glover, DM (1988) polo, a mitotic mutant of Drosophila displaying abnormal spindle poles. J. Cell. Sci. 89 ( Pt 1) 25-38 PubMed GONUTS page
  24. Mirouse, V et al. (2006) Interaction between Polo and BicD proteins links oocyte determination and meiosis control in Drosophila. Development 133 4005-13 PubMed GONUTS page
  25. Wang, H et al. (2007) Polo inhibits progenitor self-renewal and regulates Numb asymmetry by phosphorylating Pon. Nature 449 96-100 PubMed GONUTS page
  26. Gonzalez, C & Bornens, M (2000) Light, flies and cell division. Workshop: regulation of cell division in Drosophila. EMBO Rep. 1 390-3 PubMed GONUTS page
  27. Neumüller, RA et al. (2011) Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Cell Stem Cell 8 580-93 PubMed GONUTS page
  28. Ahmad, SM et al. (2012) Two forkhead transcription factors regulate the division of cardiac progenitor cells by a Polo-dependent pathway. Dev. Cell 23 97-111 PubMed GONUTS page
  29. Pek, JW et al. (2012) Polo-mediated phosphorylation of Maelstrom regulates oocyte determination during oogenesis in Drosophila. Development 139 4505-13 PubMed GONUTS page