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PMID:9813088

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Citation

Carmena, M, Riparbelli, MG, Minestrini, G, Tavares, AM, Adams, R, Callaini, G and Glover, DM (1998) Drosophila polo kinase is required for cytokinesis. J. Cell Biol. 143:659-71

Abstract

A number of lines of evidence point to a predominance of cytokinesis defects in spermatogenesis in hypomorphic alleles of the Drosophila polo gene. In the pre-meiotic mitoses, cytokinesis defects result in cysts of primary spermatocytes with reduced numbers of cells that can contain multiple centrosomes. These are connected by a correspondingly reduced number of ring canals, structures formed by the stabilization of the cleavage furrow. The earliest defects during the meiotic divisions are a failure to form the correct mid-zone and mid-body structures at telophase. This is accompanied by a failure to correctly localize the Pavarotti kinesin- like protein that functions in cytokinesis, and of the septin Peanut and of actin to be incorporated into a contractile ring. In spite of these defects, cyclin B is degraded and the cells exit M phase. The resulting spermatids are frequently binuclear or tetranuclear, in which case they develop either two or four axonemes, respectively. A significant proportion of spermatids in which cytokinesis has failed may also show the segregation defects previously ascribed to polo1 mutants. We discuss these findings in respect to conserved functions for the Polo-like kinases in regulating progression through M phase, including the earliest events of cytokinesis.

Links

PubMed PMC2148135

Keywords

Anaphase; Animals; Cell Differentiation; Cell Division/physiology; Cyclin B/metabolism; Drosophila/metabolism; Drosophila Proteins; Insect Proteins/genetics; Insect Proteins/metabolism; Male; Meiosis; Microtubule-Associated Proteins/metabolism; Mitosis; Mitotic Spindle Apparatus; Mutagenesis; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; Spermatids/metabolism; Spermatocytes/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:O16048

located_in

GO:0070938: contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O16048

located_in

GO:0032154: cleavage furrow

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O16048

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O16048

located_in

GO:0035323: male germline ring canal

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O62528

located_in

GO:0070938: contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O62528

located_in

GO:0032154: cleavage furrow

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O62528

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:O62528

located_in

GO:0035323: male germline ring canal

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PNUT

located_in

GO:0032154: cleavage furrow

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8SXT9

located_in

GO:0070938: contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8SXT9

located_in

GO:0032154: cleavage furrow

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8SXT9

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q8SXT9

located_in

GO:0035323: male germline ring canal

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VZF5

located_in

GO:0070938: contractile ring

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VZF5

located_in

GO:0032154: cleavage furrow

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VZF5

located_in

GO:0005930: axoneme

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:Q9VZF5

located_in

GO:0035323: male germline ring canal

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0051257: meiotic spindle midzone assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0030726: male germline ring canal formation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0007112: male meiosis cytokinesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0000915: actomyosin contractile ring assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0034613: cellular protein localization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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