PMID:11483958

Citation	Blower, MD and Karpen, GH (2001) The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions. Nat. Cell Biol. 3:730-9
Abstract	Centromere function requires the coordination of many processes including kinetochore assembly, sister chromatid cohesion, spindle attachment and chromosome movement. Here we show that CID, the Drosophila homologue of the CENP-A centromere-specific H3-like proteins, colocalizes with molecular-genetically defined functional centromeres in minichromosomes. Injection of CID antibodies into early embryos, as well as RNA interference in tissue-culture cells, showed that CID is required for several mitotic processes. Deconvolution fluorescence microscopy showed that CID chromatin is physically separate from proteins involved in sister cohesion (MEI-S332), centric condensation (PROD), kinetochore function (ROD, ZW10 and BUB1) and heterochromatin structure (HP1). CID localization is unaffected by mutations in mei-S332, Su(var)2-5 (HP1), prod or polo. Furthermore, the localization of POLO, CENP-meta, ROD, BUB1 and MEI-S332, but not PROD or HP1, depends on the presence of functional CID. We conclude that the centromere and flanking heterochromatin are physically and functionally separable protein domains that are required for different inheritance functions, and that CID is required for normal kinetochore formation and function, as well as cell-cycle progression.
Links	PubMed PMC3229202 Online version:10.1038/35087045
Keywords	Animals; Antibodies/pharmacology; Autoantigens; Cell Cycle/genetics; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cells, Cultured; Chromosomal Proteins, Non-Histone/genetics; Chromosomal Proteins, Non-Histone/metabolism; Chromosome Segregation/genetics; DNA/metabolism; DNA-Binding Proteins; Drosophila Proteins; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/drug effects; Embryo, Nonmammalian/metabolism; Heterochromatin/genetics; Histones/genetics; Histones/immunology; Histones/metabolism; Insect Proteins/genetics; Insect Proteins/metabolism; Interphase/genetics; Kinetochores/metabolism; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Mutation/physiology; Protein Kinases/genetics; Protein Kinases/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.