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PMID:18832073

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Citation

Archambault, V, D'Avino, PP, Deery, MJ, Lilley, KS and Glover, DM (2008) Sequestration of Polo kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by phosphorylation at a CDK site in mitosis. Genes Dev. 22:2707-20

Abstract

The conserved Polo kinase controls multiple events in mitosis and cytokinesis. Although Polo-like kinases are regulated by phosphorylation and proteolysis, control of subcellular localization plays a major role in coordinating their mitotic functions. This is achieved largely by the Polo-Box Domain, which binds prephosphorylated targets. However, it remains unclear whether and how Polo might interact with partner proteins when priming mitotic kinases are inactive. Here we show that Polo associates with microtubules in interphase and cytokinesis, through a strong interaction with the microtubule-associated protein Map205. Surprisingly, this interaction does not require priming phosphorylation of Map205, and the Polo-Box Domain of Polo is required but not sufficient for this interaction. Moreover, phosphorylation of Map205 at a CDK site relieves this interaction. Map205 can stabilize Polo and inhibit its cellular activity in vivo. In syncytial embryos, the centrosome defects observed in polo hypomorphs are enhanced by overexpression of Map205 and suppressed by its deletion. We propose that Map205-dependent targeting of Polo to microtubules provides a stable reservoir of Polo that can be rapidly mobilized by the activity of Cdk1 at mitotic entry.

Links

PubMed PMC2559908 Online version:10.1101/gad.486808

Keywords

Amino Acid Sequence; Animals; Animals, Genetically Modified; Binding Sites; CDC2 Protein Kinase/metabolism; Cytokinesis; Drosophila Proteins/chemistry; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Female; Interphase; Microtubule-Associated Proteins/chemistry; Microtubule-Associated Proteins/genetics; Microtubule-Associated Proteins/metabolism; Microtubules/metabolism; Mitosis; Models, Biological; Molecular Sequence Data; Phosphorylation; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases/chemistry; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:MA205

involved_in

GO:0000278: mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:MA205

involved_in

GO:0000278: mitotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0003124

P

Seeded From UniProt

complete

DROME:POLO

located_in

GO:0005819: spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

located_in

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

located_in

GO:0000776: kinetochore

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

located_in

GO:0005874: microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

located_in

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

part_of

GO:0005813: centrosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

part_of

GO:0005819: spindle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0000278: mitotic cell cycle

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:POLO

part_of

GO:0005635: nuclear envelope

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

part_of

GO:0005874: microtubule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

part_of

GO:0000776: kinetochore

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:POLO

involved_in

GO:0000278: mitotic cell cycle

ECO:0000316: genetic interaction evidence used in manual assertion

FB:FBgn0002645

P

Seeded From UniProt

complete


See also

References

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