Category:Team Translators

Figure 7

Figure 8

in figure 6A. neural tube closure defects and exencephaly is showed. this was done by creating Tctn2 null mice with a mixed background of 129/Bl6. presenting Tctn2 mutants

in figure 4C. silencing SIL1 resulted in delay migration. Neuronal migration was monitored by pCAG-EGFP, which is a reporter and staining for GFP.

Figure 1.C. demonstrates that BBD 18 prevents the production/expression of OspC in B. bugorferi wild-type strains. Figure 1.F. shows that in ∆rpos and wild type/faltBp-bbd 18 strains do not have the same brands as wild-type. Hence, the genes are RpoS-dependent. This means that the present of BBD 18 represses the expression of RpoS-dependent genes. Figure 2 is the data of quantitative reverse transcriptase PCR to demonstrate that BBD 18 repression is at the level of transcription.

Figure 3.b. and 3.c. indicate that four HIRIP3 siRNA causes HR defect. Even though these HIRIP3 siRNA are also correlated with off target Rad51 depletion, only siHIRIP3-5 had 7 nucleotide seed match to 3‘UTR Rad51 off-targeting. Therefore, HR defects are 3-fold enriched for antisense seed sequence complementary to Rad51. However, Figure 3.b. also indicates three siRNAs defect HR depleted Rad51 without full seed complementary to the 3’UTR. Therefore, HIRIP3 siRNAs can change the mechanisms of Rad51 off-targeting.

The RNA binding region of RBMX is really important for HR. However, it is not for DNA lesions. This tells us that RBMX promotes HR trough protein expression due to splicing events. The PCR results shows that RBMX siRNAs also decreases BRCA2 levels in a manner that siRNA-resistant FHA-RBMX is involve (Figure 6d-e). RBMX does not have an effect on PALB2, BRCA1 and RPA2.

The graph shown in Figure 4.a. tells us that RBMX acts as a recruiter. Hence, it is needed for resistance to DNA damage since it will recruit all the factors necessary for DNA damage repair. When RBMX leaves, it accumulates at sites of DNA in a poly (ADP-Ribose) polymerase (PARP1) dependent manner called anti-stripe (Figure 4 b-c).