It is now the 1st OPEN Period for CACAO Fall 2017! It will end on Sunday September 24, 2017 at 11:59 pm CDT
This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:Team Phergalicious

From GONUTS
Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBPSPP:O48472Mcgillje, Team Phergalicious2017-04-05 10:07:16 CDTGO:0020002 host cell plasma membrane (C)PMID:27825035IDA

In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp26 shows that the protein product was detected at much higher levels in the cell membrane, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp26.

challenge
flagged9CAUD:I6P9Q0Alickovicl, Team Phergalicious2017-04-19 09:26:58 CDTGO:0016021 integral component of membrane (C)PMID:26873847IDA

Figure 3d shows the expression of HolGH15 over 10 minute intervals after western blotting was performed. The E. coli BL21-CodonPlus cells harbouring pET-28a-HOLGH15 were induced with IPTG to detect HolGH15. The result demonstrated the presence of a band of about 21 kDa with consistent size with the predicted mass of HolGH15. The band was detected 10 minutes after the induction with IPTG. Figure 3b: Transmission Electron Microscopy observations showed damage and disruption in the induced E. coli cells. The result was the release of cellular contents and showed apperance simillar to empty, non-living bacterial envelopes of Gram-negative bacteria.

challenge
acceptableBPSPP:HOL24Mcgillje, Team Phergalicious2017-04-19 09:38:02 CDTGO:0020002 host cell plasma membrane (C)PMID:27825035IDA

In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp 24.1 shows that the holin-like protein of Bacillus phage SPP1 was detected at much higher levels in the cell membrane after cell fractionation, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp24.1, with no band visible in the cytoplasmic fraction.

challenge
flaggedBPMD2:HOLINVarillaz, Team Phergalicious2017-04-26 19:56:16 CDTGO:0020002 host cell plasma membrane (C)PMID:26471254IDA

Figure 2F shows a western blot performed after IPTG induction to detect the presence of HolFL by using anti-6xHis antibodies and Gp10 with anti-gp10 antibodies. pSKHol+gp10 shows higher expression between 42kDa and above, it also shows higher expression between 22kDa and 14kDa.

challenge

What do the icons mean in the status column?

assessment icons.jpg

Pages in category "Team Phergalicious"

The following 3 pages are in this category, out of 3 total.


Jump to pages starting with: A M V