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BPSPP:HOL24
Contents
Species (Taxon ID) | Bacillus phage SPP1 (Bacteriophage SPP1). (10724) | |
Gene Name(s) | 24.1 | |
Protein Name(s) | Holin-like protein 24.1 (ECO:0000312 with EMBL:CAA66517.2)
Gene product 24.1 (ECO:0000305) gp24.1 (ECO:0000305) | |
External Links | ||
UniProt | O48470 | |
EMBL | X97918 | |
PIR | T42310 | |
RefSeq | NP_690701.2 | |
TCDB | 1.E.31.1.13 | |
GeneID | 955351 | |
KEGG | vg:955351 | |
Proteomes | UP000002559 | |
GO | GO:0016021 | |
InterPro | IPR019715 | |
Pfam | PF10779 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0020002 |
host cell plasma membrane |
ECO:0000314 |
C |
In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp 24.1 shows that the holin-like protein of Bacillus phage SPP1 was detected at much higher levels in the cell membrane after cell fractionation, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp24.1, with no band visible in the cytoplasmic fraction. |
complete | |||||
GO:0033644 |
host cell membrane |
ECO:0000315 |
C |
Figure 3C illustrates the alignment of nucleotide sequence of the early promoters PE1, PE2,and PE3 in comparison to that of PE5 in the SPP1 genome sequence. As the cell grows the B.subtilis in seen to not have an effect on its growth. The occurrence of holin had little effect on the development of the cell. |
complete | |||||
GO:0039662 |
host cell outer membrane |
ECO:0000314 |
C |
Bacillus phage SPP1 (Bacteriophage SPP1) holin-like protein 24.1 (gp24.1) As shown in Figure 4, the holin-like protein gp24.1 localizes to the cytosplasmic membrane. |
complete | |||||
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
UniProtKB-KW:KW-1032 |
C |
Seeded From UniProt |
complete | ||
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0019835 |
cytolysis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 Fernandes, S & São-José, C (2017) Probing the function of the two holin-like proteins of bacteriophage SPP1. Virology 500 184-189 PubMed GONUTS page