User:Mcgillje

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BPSPP:O48472	2017-04-05 10:07:16 CDT	GO:0020002 host cell plasma membrane (C)	PMID:27825035	ECO:0000314 direct assay evidence used in manual assertion	In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp26 shows that the protein product was detected at much higher levels in the cell membrane, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp26.	challenge
acceptable	BPSPP:HOL24	2017-04-19 09:38:02 CDT	GO:0020002 host cell plasma membrane (C)	PMID:27825035	ECO:0000314 direct assay evidence used in manual assertion	In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp 24.1 shows that the holin-like protein of Bacillus phage SPP1 was detected at much higher levels in the cell membrane after cell fractionation, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp24.1, with no band visible in the cytoplasmic fraction.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Mcgillje

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Mcgillje

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