User:Alickovicl

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Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
flagged	9CAUD:I6P9Q0	2017-04-19 09:26:58 CDT	GO:0016021 integral component of membrane (C)	PMID:26873847	ECO:0000314 direct assay evidence used in manual assertion	Figure 3d shows the expression of HolGH15 over 10 minute intervals after western blotting was performed. The E. coli BL21-CodonPlus cells harbouring pET-28a-HOLGH15 were induced with IPTG to detect HolGH15. The result demonstrated the presence of a band of about 21 kDa with consistent size with the predicted mass of HolGH15. The band was detected 10 minutes after the induction with IPTG. Figure 3b: Transmission Electron Microscopy observations showed damage and disruption in the induced E. coli cells. The result was the release of cellular contents and showed apperance simillar to empty, non-living bacterial envelopes of Gram-negative bacteria.	challenge

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