BPMD2:HOLIN

Species (Taxon ID)	Mycobacterium phage D29 (Mycobacteriophage D29). (28369)
Gene Name(s)	11
Protein Name(s)	Holin (ECO:0000303 with PMID:26471254) Gene product 11 (ECO:0000305) gp11 (ECO:0000305)
External Links
UniProt	O64204
EMBL	AF022214
PIR	H72800
RefSeq	NP_046826.1
SMR	O64204
TCDB	1.E.36.1.7
GeneID	1261605
KEGG	vg:1261605
OrthoDB	VOG090001E7
Proteomes	UP000002131
GO	GO:0020002 GO:0016021 GO:0044660
InterPro	IPR032121
Pfam	PF16081

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0020002	host cell plasma membrane	PMID:26471254	ECO:0000314			C		Figure 2F shows a western blot performed after IPTG induction to detect the presence of HolFL by using anti-6xHis antibodies and Gp10 with anti-gp10 antibodies. pSKHol+gp10 shows higher expression between 42kDa and above, it also shows higher expression between 22kDa and 14kDa.	complete CACAO 12894
	GO:0044660	cytolysis by virus via pore formation in host cell membrane	PMID:26471254	ECO:0000315			P		Mycobacterium phage D29 gene gp11 codes for the protein holin. The protein allows phage D29 to kill both Mycobacterium smegmatis and Escherichia coli by creating pores in the host membrane. The expression of the entire gp11 gene (using vectors) decreased the growth of E. coli significantly (Fig 2A and presence of gp11 confirmed by Western Blot in 2B). The expression of whole gp11 also decreased the growth of M. smegmatis (Fig 2C). Shortened versions Hol68 and Hol93, with amino acids cut off the C-terminal end, did not affect E. coli bacterial growth but Hol116 had a similar reduction in bacterial growth to full length holin protein (Fig. 3B). Figure 3C confirms the presence of holin in all shortened versions and compares them to full length holin. Spot assays confirm the data found in figure 3B because Hol116 showed little to no growth, whereas Hol68 and Hol93 show a significantly larger amount of growth compared to full length holin and Hol116 (Fig. 3D). The spot test of Hol116 also looked very similar to full length holin (Fig. 3D). This indicates that the N-terminal, up through amino acid 116, is important in the function of holin to cause bacterial cell death. The protein must be greater than 93 amino acids long to still contain the function of holin (Fig. 3, all).	complete CACAO 13102
involved_in	GO:0044660	cytolysis by virus via pore formation in host cell membrane	PMID:26471254	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0019835	cytolysis	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0204	P		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
part_of	GO:0033644	host cell membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1043	C		Seeded From UniProt	complete
part_of	GO:0020002	host cell plasma membrane	GO_REF:0000037 GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1032 UniProtKB-KW:KW-1030 UniProtKB-SubCell:SL-0373	C		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C		Seeded From UniProt	complete
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References

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