Category:Team Noisy Gene Expression

Table 1 shows the results of whether or not the expression of SQD1 homolog of C. reinhardtii in a disruptant as to the sqdB gene of Synechococcus sp. CC7942 complements the SQDG defective phenotype. The data compiled showed accumulation of SQDG up to 2.9% of total lipid with a significant

level of the transcript of the SQD1 cDNA, confirming that the cDNA indeed encodes UDP-sulfoquinovose synthase.

Analysis of mutant strains along with comparison to similar genes within organism point to CYP82E4v1 being responsible for demethylation of nicotine.

Comparison of mutant strains versus wild type show that LUT1 exclusively encodes for ε-hydroxylase activity in photosynthetic tissues and carries this function out as part of carotenoid synthesis.

Figure 4 shows an assay of the Pac enzyme with pur6 products. Pac is shown to be highly specific interactign with only puromycin carbon backbones. Based on the interaction of pur6 and the Pac enzyme, and the specificity of the enzyme that pur6 is involved in the puromycin synthesis.

Table 2 shows significant differences of Vitamin E concentrations, with a major reduction of product in the mutant strain as compared to wild type. Further tests show that Vitamin E concentrations were the only substance effected showing its relation to vitamin E biosynthesis.

Figure 7 shows that analysis of desA mutants is essential to the O demethylation activity of syringate.

Figure 2 has real-time PCR results that show a 7x increase in Hash4 activity in fetal skin compared to adult skin, indicting an involvement in skin development.

Table 1 summarizes the use of an antibody to confirm that ckx1 encodes the cytokinin oxidase. Figure 5 shows that the oxidase has a spectrum characteristic of a flavoprotein oxidoreductase.