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PMID:10049708
| Citation |
Morris, RO, Bilyeu, KD, Laskey, JG and Cheikh, NN (1999) Isolation of a gene encoding a glycosylated cytokinin oxidase from maize. Biochem. Biophys. Res. Commun. 255:328-33 |
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| Abstract |
The major cytokinin oxidase in immature maize kernels was purified to homogeneity. Selected tryptic peptides were used to design degenerate oligonucleotide primers for PCR isolation of a fragment of the oxidase gene. Hybridization of the PCR fragment to a maize genomic library allowed isolation of a full-length cytokinin oxidase gene (ckx1). The gene encodes a protein of approximately 57 kDa that possesses a signal peptide, eight consensus N-glycosylation sequences and a consensus FAD binding sequence. Expression of ckx1 in Pichia caused secretion of active glycosylated cytokinin oxidase that contains a substrate-reducible FAD. The gene displays sequence homology with a putative oxidoreductase from Arabidopsis thaliana and with the fas5 gene from Rhodococcus fascians. |
| Links |
PubMed Online version:10.1006/bbrc.1999.0199 |
| Keywords |
Amino Acid Sequence; Cytokinins/chemistry; Cytokinins/genetics; Genes, Plant; Glycosylation; Molecular Sequence Data; Oxidoreductases/chemistry; Oxidoreductases/genetics; Oxidoreductases/isolation & purification; Oxidoreductases/metabolism; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Substrate Specificity; Zea mays/enzymology; Zea mays/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019139: cytokinin dehydrogenase activity |
ECO:0000314: |
F |
Table 1 summarizes the use of an antibody to confirm that ckx1 encodes the cytokinin oxidase. Figure 5 shows that the oxidase has a spectrum characteristic of a flavoprotein oxidoreductase. |
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See also
References
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