GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Angel Rubio, M, Barrado, P, Carlos Espinosa, J, Jiménez, A and Fernández Lobato, M (2004) The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase. FEBS Lett. 577:371-5
The pur6 gene of the puromycin biosynthetic gene (pur) cluster from Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking L-tyrosine to both 3'-amino-3'-deoxyadenosine and N6,N6-dimethyl-3'-amino-3'-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from Streptomyces lividans or Escherichia coli transformants that expressed pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway.
Chemistry, Physical/methods; Escherichia coli/genetics; Gene Deletion; Gene Expression; Genes, Bacterial; Kinetics; Molecular Structure; Multigene Family; Plasmids; Puromycin/biosynthesis; Puromycin Aminonucleoside/chemistry; Puromycin Aminonucleoside/metabolism; Streptomyces/genetics; Streptomyces/metabolism; Streptomyces lividans/genetics
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:0043638: puromycin biosynthetic process||
Figure 4 shows an assay of the Pac enzyme with pur6 products. Pac is shown to be highly specific interactign with only puromycin carbon backbones. Based on the interaction of pur6 and the Pac enzyme, and the specificity of the enzyme that pur6 is involved in the puromycin synthesis.
See Help:References for how to manage references in GONUTS.