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PMID:11418103
| Citation |
Schledz, M, Seidler, A, Beyer, P and Neuhaus, G (2001) A novel phytyltransferase from Synechocystis sp. PCC 6803 involved in tocopherol biosynthesis. FEBS Lett. 499:15-20 |
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| Abstract |
The deduced polypeptide sequence of open reading frame slr1736 reveals homology to chlorophyll synthase and 1,4-dihydroxy-2-naphthoic acid phytyltransferase in Synechocystis sp. strain PCC 6803. In tocopherol and plastoquinone biosynthesis, a condensation reaction mechanistically similar to that of these two enzymes is performed. To analyze the function of this novel prenyltransferase, a deletion mutant of slr1736 was generated by homologous recombination. The mutant showed a markedly decreased tocopherol content, while plastoquinone levels remained unchanged. Since the aromatic precursor homogentisic acid accumulated in the mutant, the function of the enzyme was proven to be a novel tocopherol phytyltransferase. |
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| Keywords |
Alkyl and Aryl Transferases/chemistry; Alkyl and Aryl Transferases/genetics; Alkyl and Aryl Transferases/metabolism; Amino Acid Sequence; Chromatography, High Pressure Liquid; Cloning, Molecular; Cyanobacteria/enzymology; Cyanobacteria/genetics; Gene Deletion; Homogentisic Acid/metabolism; Molecular Sequence Data; Open Reading Frames/genetics; Pigments, Biological/metabolism; Plastoquinone/metabolism; Sequence Alignment; Vitamin E/biosynthesis; Vitamin E/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0010189: vitamin E biosynthetic process |
ECO:0000315: |
P |
Table 2 shows significant differences of Vitamin E concentrations, with a major reduction of product in the mutant strain as compared to wild type. Further tests show that Vitamin E concentrations were the only substance effected showing its relation to vitamin E biosynthesis.
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complete | ||||
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involved_in |
GO:0010189: vitamin E biosynthetic process |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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