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Category:Team Green Machine

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableBACIU:J7JPD5Dbilling, Team Green Machine2013-10-27 14:50:40 CDTGO:0008134 transcription factor binding (table 3)PMID:10094682IMP: Inferred from Mutant Phenotype
challenge
updatedbyinstructorBACTK:B5SRV2Dbilling, Team Green Machine2013-10-27 14:22:59 CDTGO:0003867 4-aminobutyrate transaminase activity (F)PMID:19854901ECO:0000315 mutant phenotype evidence used in manual assertion

fig. 3

challenge
acceptableBACSU:GABTDbilling, Team Green Machine2013-10-27 13:48:47 CDTGO:0003867 4-aminobutyrate transaminase activity (F)PMID:12123465ECO:0000315 mutant phenotype evidence used in manual assertion

table1. deletion of gabT gene causes cell to be unable to use GABA as sole nitrogen source.

challenge
acceptableECOLI:CSPCBblakeley, Team Green Machine2013-10-25 14:53:37 CDTGO:0051087 chaperone binding (F)PMID:24148697ECO:0000314 direct assay evidence used in manual assertion

Figure 5 of the paper shows that CspC levels decrease when GroEL is overexpressed.

challenge
updatedbyinstructorHERSS:D8IWL7Dbilling, Team Green Machine2013-10-09 22:35:08 CDTGO:0010628 positive regulation of gene expression (P)PMID:23996052ECO:0000315 mutant phenotype evidence used in manual assertion

fig 4b

challenge
updatedbyinstructorHERSS:D8J1B0Dbilling, Team Green Machine2013-10-09 22:33:55 CDTGO:0010628 positive regulation of gene expression (P)PMID:23996052ECO:0000315 mutant phenotype evidence used in manual assertion

fig 4b

challenge
requireschangesPHOLE:A7KQM2Dbilling, Team Green Machine2013-10-08 13:41:06 CDTGO:0048255 mRNA stabilization (P)PMID:8941351ECO:0000314 direct assay evidence used in manual assertion

fig 5. bioluminoassays show the loops involved in mRNA stability

challenge
unacceptablePLAF7:Q8I377Dbilling, Team Green Machine2013-10-08 00:00:03 CDTGO:0051085 chaperone mediated protein folding requiring cofactor (P)PMID:23994135ECO:0000315 mutant phenotype evidence used in manual assertion

Fig4. ClpB1 is required to activate proper folding of luciferase after heat shock conditions

challenge
acceptableECO7I:B7NQF7Dbilling, Team Green Machine2013-09-27 15:55:39 CDTGO:0008378 galactosyltransferase activity (F)PMID:15625181ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1: mutant wbbD resulted in low galactosyltransferase activity in E. coli. This shows that wbbD is involved in the Gal transfer reaction.

challenge
updatedbyinstructorSALPA:Q5PDX1Dbilling, Team Green Machine2013-09-27 10:29:55 CDTGO:0045429 positive regulation of nitric oxide biosynthetic process (P)PMID:24055826ECO:0000314 direct assay evidence used in manual assertion

Fig 1: using Griess reagent assay, it was found that increased concentration of BaeR protein resulted in increased production of Nitric oxide

challenge
updatedbyinstructorVIBAN:VANRDbilling, Team Green Machine2013-09-25 21:36:17 CDTGO:1901336 lactone biosynthetic process (P)PMID:9139920ECO:0000315 mutant phenotype evidence used in manual assertion

Fig1A: A vanR negative strain of Vibrio anguillarum was created using a suicide plasmid containing chloramphenicol resistance gene; this resulted in a mutant phenotype (non-functioning vanR). As a result of a non-functioning vanR, there was no ODHL produced. This indicated that vanR acts as the transcriptional activator of vanI which is involved in ODHL synthesis (the auto-inducer molecule for this organism).

challenge
updatedbyinstructorVIBAN:VANIDbilling, Team Green Machine2013-09-25 21:21:50 CDTGO:1901336 lactone biosynthetic process (P)PMID:9139920ECO:0000315 mutant phenotype evidence used in manual assertion

Fig2. In-frame deletion mutagenesis was carried out on vanI; this resulted in no ODHL (N-(3-oxodecanoyl)-L-homoserine lactone) production. This shows that vanI is responsible for ODHL synthesis.

challenge

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Pages in category "Team Green Machine"

The following 4 pages are in this category, out of 4 total.


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