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PMID:12123465
| Citation |
Belitsky, BR and Sonenshein, AL (2002) GabR, a member of a novel protein family, regulates the utilization of gamma-aminobutyrate in Bacillus subtilis. Mol. Microbiol. 45:569-83 |
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| Abstract |
The Bacillus subtilis ycnG (gabT) and ycnH (gabD) genes were shown to encode gamma-aminobutyrate (GABA) aminotransferase and succinic semi-aldehyde dehydrogenase, respectively, and to form a GABA-inducible operon. Null mutations in gabT, gabD or the divergently transcribed ycnF (gabR) gene blocked the utilization of GABA as sole nitrogen source. GabR proved to be a transcriptional activator of the gabTD operon and a negative autoregulator. The target of GabR action was localized to an 87 bp region that includes both gabR and gabT promoters. GabR is a member of a novel but widespread family of chimeric bacterial proteins that have apparent DNA-binding and aminotransferase domains. Mutations in conserved residues of the putative aminotransferase domain abolished GabR function as a transcriptional activator, but did not affect its activity as a negative autoregulator. |
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| Keywords |
4-Aminobutyrate Transaminase/genetics; 4-Aminobutyrate Transaminase/physiology; Aldehyde Oxidoreductases/genetics; Aldehyde Oxidoreductases/physiology; Amino Acid Sequence; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/physiology; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/genetics; Cloning, Molecular; Gene Deletion; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Multigene Family; Nitrogen/metabolism; Operon/genetics; Promoter Regions, Genetic/genetics; Repressor Proteins/genetics; Repressor Proteins/physiology; Sequence Alignment; Sequence Homology, Amino Acid; Succinate-Semialdehyde Dehydrogenase; Trans-Activators/genetics; Trans-Activators/physiology; gamma-Aminobutyric Acid/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003867: 4-aminobutyrate transaminase activity |
ECO:0000315: |
F |
table1. deletion of gabT gene causes cell to be unable to use GABA as sole nitrogen source. |
complete | ||||
|
enables |
GO:0003867: 4-aminobutyrate transaminase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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