PMID:8941351

Citation	Lin, JW, Chao, YF and Weng, SF (1996) Nucleotide sequence and functional analysis of the luxE gene encoding acyl-protein synthetase of the lux operon from Photobacterium leiognathi. Biochem. Biophys. Res. Commun. 228:764-73
Abstract	Nucleotide sequence of the luxE gene GenBank Accession No. U66407 from Photobacterium leiognathi PL741 has been determined, and the amino acid sequence of acyl-protein synthetase encoded by the luxE gene is deduced. Nucleotide sequence reveals that the luxE gene encodes acyl-protein synthetase, which is a component of the fatty acid reductase complex that is responsible for converting fatty acid to aldehyde as substrate in the luciferase-catalyzed bioluminescence reaction. The acyl-protein synthetase encoded by the luxE gene has a calculated M, 43,128 and comprises 373 amino acid residues. Alignment and comparison of acyl-protein synthetases from P. leiognathi, P. phosphoreum, Vibrio fischeri, V. harveyi and Xenorhabdus luminescens shows that they are homologous; there is 75.5% homologous (44.2% identity and 31.3% similarity) among these species. Functional analysis illustrates that the specific segment sequence lying before or in the luxE gene might from potential loops omega o omega e1, omega e2 as mRNA stability loop and/or for sub-regulation by alternative modulation in the lux operon. The gene order of the luxE gene in the lux and the lum operons is<--ter-lumQ-lumP-R&R-luxC-luxD-luxA-luxB -luxN-luxE-->(R&R: regulatory region; ter; transcriptional terminator), whereas the R&R is the regulatory region for the lum and the lux operons, and ter is the transcriptional terminator for the lum operon.
Links	PubMed Online version:10.1006/bbrc.1996.1729
Keywords	Acyltransferases/genetics; Acyltransferases/metabolism; Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA, Bacterial; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Molecular Sequence Data; Nucleic Acid Conformation; Operon; Photobacterium/enzymology; Photobacterium/genetics; Sequence Homology, Amino Acid; Species Specificity
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
PHOLE:A7KQM2		GO:0048255: mRNA stabilization	ECO:0000314:		P		fig 5. bioluminoassays show the loops involved in mRNA stability	complete CACAO 8784

References

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