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Category:Team Blue B 2019

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBPPRD:ENLYSPMariano, Team Blue B 20192019-04-14 16:50:02 CDTGO:0044659 cytolysis by virus of host cell (P)PMID:16030234ECO:0006006 electrophysiology assay evidence used in manual assertion

Figure 5 shows the electrochemical assays performed on recombinant E. coli to determine the physiological effects of PRD1 endolysin (pMG118) involved in viral cytolysis. Host cell permeability (K+ and TPP+), cell growth and cell cytolysis (A550) were measured. Fig. 5a exhibited cell growth that steadily increased but never decreased, and variable changes in permeability. There was a peaked influx and efflux of K+, a decrease in TPP+, and steady, increased A550. The absence of endolysin and the presence of holin (pGZ9) failed to rupture cell membrane. Fig. 5e exhibited cytolysis with co-expressed holin and endolysin. Permeability and cell growth increased and peaked, but decreased dramatically below the initial baseline. The initial influx and latter efflux of K+, TPP+, and A550 values displayed how endolysin works to rupture host membrane when simultaneously present with holin in PRD1 for this synergistic effect.

challenge
acceptableBPPRD:HOLINPMariano, Team Blue B 20192019-03-24 16:51:20 CDTGO:0044659 cytolysis by virus of host cell (P)PMID:16030234ECO:0006006 electrophysiology assay evidence used in manual assertion

Figure 5 shows the electrochemical assays performed on recombinant E. coli to determine the physiological effects of PRD1 holin (pGZ9) involved in viral cytolysis. Host cell permeability (K+ and TPP+), cell growth and cell cytolysis (A550) were measured. Fig. 5c exhibited steady, increased permeability and growth but no cytolysis. The absence of holin and the presence of endolysin (pMG118) failed to rupture cell membrane. K+, TPP+, and A550 grew in concentration and did not decrease. Fig. 5e exhibited cytolysis with co-expressed holin and endolysin. Permeability and cell growth increased and peaked, but decreased dramatically below the initial baseline. The initial influx and latter efflux of K+, TPP+, and A550 values displayed how holin works to rupture host membrane when simultaneously present with endolysin in PRD1 for this synergistic effect.

challenge
acceptableLAMBD:TERLTNaqvi, Team Blue B 20192019-03-11 13:57:35 CDTGO:0019073 viral DNA genome packaging (P)PMID:11866517ECO:0007101 southern hybridization evidence used in manual assertion

Researchers wanted to show that gpA (found in the terminase large subunit) helps package phage DNA. A lambda bacteriophage was used in this experiment and various gpA mutants were cut (with a restriction endonuclease) and cloned into this phage; the amount of DNA packaged was determined experimentally by quantifying it in vivo. Table 5 shows how a normal lambda-P1 Sam7 phage with no mutated gpA was able to package 100% of its DNA. But when the same phage contained any type of mutated gpA, the percentage of DNA packaged decreased dramatically.

challenge

Pages in category "Team Blue B 2019"

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