User:PMariano

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BPPRD:HOLIN	2019-03-24 16:51:20 CDT	GO:0044659 cytolysis by virus of host cell (P)	PMID:16030234	ECO:0006006 electrophysiology assay evidence used in manual assertion	Figure 5 shows the electrochemical assays performed on recombinant E. coli to determine the physiological effects of PRD1 holin (pGZ9) involved in viral cytolysis. Host cell permeability (K+ and TPP+), cell growth and cell cytolysis (A550) were measured. Fig. 5c exhibited steady, increased permeability and growth but no cytolysis. The absence of holin and the presence of endolysin (pMG118) failed to rupture cell membrane. K+, TPP+, and A550 grew in concentration and did not decrease. Fig. 5e exhibited cytolysis with co-expressed holin and endolysin. Permeability and cell growth increased and peaked, but decreased dramatically below the initial baseline. The initial influx and latter efflux of K+, TPP+, and A550 values displayed how holin works to rupture host membrane when simultaneously present with endolysin in PRD1 for this synergistic effect.	challenge
acceptable	BPPRD:ENLYS	2019-04-14 16:50:02 CDT	GO:0044659 cytolysis by virus of host cell (P)	PMID:16030234	ECO:0006006 electrophysiology assay evidence used in manual assertion	Figure 5 shows the electrochemical assays performed on recombinant E. coli to determine the physiological effects of PRD1 endolysin (pMG118) involved in viral cytolysis. Host cell permeability (K+ and TPP+), cell growth and cell cytolysis (A550) were measured. Fig. 5a exhibited cell growth that steadily increased but never decreased, and variable changes in permeability. There was a peaked influx and efflux of K+, a decrease in TPP+, and steady, increased A550. The absence of endolysin and the presence of holin (pGZ9) failed to rupture cell membrane. Fig. 5e exhibited cytolysis with co-expressed holin and endolysin. Permeability and cell growth increased and peaked, but decreased dramatically below the initial baseline. The initial influx and latter efflux of K+, TPP+, and A550 values displayed how endolysin works to rupture host membrane when simultaneously present with holin in PRD1 for this synergistic effect.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by PMariano

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	PMariano, Team Blue B 2019	BPPRD:HOLIN	GO:0044659 - viral release from host cell by cytolysis (P)	PMID:16030234	ECO:0006006 electrophysiology assay evidence used in manual assertion	challenge	C: 3
requireschanges	CXie, Team Red B 2019	RHIME:Q92RW9	GO:0004673 - protein histidine kinase activity (F)	PMID:17237174	ECO:0005641 IMP: Inferred from Mutant Phenotype	challenge	C: 2
acceptable	PMariano, Team Blue B 2019	BPPRD:ENLYS	GO:0044659 - viral release from host cell by cytolysis (P)	PMID:16030234	ECO:0006006 electrophysiology assay evidence used in manual assertion	challenge	C: 4

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