BPPRD:HOLIN

Species (Taxon ID)	Enterobacteria phage PRD1 (Bacteriophage PRD1). (10658)
Gene Name(s)	XXXV
Protein Name(s)	Holin Protein P35
External Links
UniProt	Q3T4L9
EMBL	AY848689
RefSeq	YP_001542615.1
TCDB	1.E.5.1.1
GeneID	5729504
KEGG	vg:5729504
OrthoDB	VOG090001GZ
Proteomes	UP000002143
GO	GO:0033644 GO:0016021 GO:0003824 GO:0019835 GO:0042742 GO:0008152 GO:0019076
InterPro	IPR032126
Pfam	PF16083

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0044659	cytolysis by virus of host cell	PMID:16030234	ECO:0006006			P		Figure 5 shows the electrochemical assays performed on recombinant E. coli to determine the physiological effects of PRD1 holin (pGZ9) involved in viral cytolysis. Host cell permeability (K+ and TPP+), cell growth and cell cytolysis (A550) were measured. Fig. 5c exhibited steady, increased permeability and growth but no cytolysis. The absence of holin and the presence of endolysin (pMG118) failed to rupture cell membrane. K+, TPP+, and A550 grew in concentration and did not decrease. Fig. 5e exhibited cytolysis with co-expressed holin and endolysin. Permeability and cell growth increased and peaked, but decreased dramatically below the initial baseline. The initial influx and latter efflux of K+, TPP+, and A550 values displayed how holin works to rupture host membrane when simultaneously present with endolysin in PRD1 for this synergistic effect.	complete CACAO 13618
	GO:0019835	cytolysis	PMID:12813073	ECO:0000315			P		Figure 3a. shows Phage PRD1 sus715 amber mutant shows loss of cytolysis phenotype is restored with bacteriophage lambda gene S105. The filled circle line shows sus715 mutant without induction - showing the lack of lysis with the mutant. The filled square shows the sus715 mutant in the presence of lambda holin induced with the addition of a sugar IPTG, and cytolysis is restored. The filled triangle shows the wild type phage and how they would expect the gene to respond without a mutation.	complete CACAO 12604
enables	GO:0003824	catalytic activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081	F		Seeded From UniProt	complete
involved_in	GO:0019835	cytolysis	GO_REF:0000037 GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081 UniProtKB-KW:KW-0204	P		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
involved_in	GO:0008152	metabolic process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081	P		Seeded From UniProt	complete
involved_in	GO:0042742	defense response to bacterium	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081	P		Seeded From UniProt	complete
part_of	GO:0033644	host cell membrane	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1043 UniProtKB-SubCell:SL-0380	C		Seeded From UniProt	complete
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