BPPRD:ENLYS

Species (Taxon ID)	Enterobacteria phage PRD1 (Bacteriophage PRD1). (10658)
Gene Name(s)	XV
Protein Name(s)	Endolysin Beta-1,4-N-acetylmuramidase Lysozyme Lytic enzyme Muramidase Protein P15
External Links
UniProt	P13559
EMBL	X14980 M22161 AY848689
PIR	S03568
RefSeq	NP_040683.1
SMR	P13559
GeneID	1260935
KEGG	vg:1260935
Proteomes	UP000002143
GO	GO:0055036 GO:0003796 GO:0051672 GO:0044277 GO:0019835 GO:0044659 GO:0042742
InterPro	IPR024408 IPR016284
Pfam	PF11860
PIRSF	PIRSF001069

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0033922	peptidoglycan beta-N-acetylmuramidase activity	PMID:7925454	ECO:0000314			F		In figure 3, samples of cell walls were combined with the enzyme P15, hydrolyzed with HCl, and chromatographed on a column. Eluted fractions were then tested for radioactivity. Only the sample with [3H]muramicitol was seen only in the tubes with P15 and cell walls, not in any of the control. This meant that P15 bound to [3H]muramicitol and hydrolyzed it, which would lead to cell death. It had previously been discovered that adding p15 to a tube of E. coli would decrease the turbidity of the tube by killing the cells. Figure 3 was just to show the nature of the cells' death.	complete CACAO 13267
involved_in	GO:0044277	cell wall disassembly	PMID:7925454	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0055036	virion membrane	PMID:11741849	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
involved_in	GO:0044659	obsolete catabolism by organism of cell wall peptidoglycan in other organism	PMID:11741849	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0044659	viral release from host cell by cytolysis	PMID:10931330	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0003796	lysozyme activity	PMID:7925454	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0003796	lysozyme activity	PMID:10931330	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0044659	cytolysis by virus of host cell	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR016284	P		Seeded From UniProt	complete
enables	GO:0003796	lysozyme activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.2.1.17	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
enables	GO:0016798	hydrolase activity, acting on glycosyl bonds	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0326	F		Seeded From UniProt	complete
involved_in	GO:0008152	metabolic process	GO_REF:0000037 GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081 UniProtKB-KW:KW-0326	P		Seeded From UniProt	complete
part_of	GO:0019012	virion	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0946	C		Seeded From UniProt	complete
involved_in	GO:0019835	cytolysis	GO_REF:0000037 GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0204 UniProtKB-KW:KW-0081	P		Seeded From UniProt	complete
involved_in	GO:0042742	defense response to bacterium	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081	P		Seeded From UniProt	complete
enables	GO:0003824	catalytic activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0081	F		Seeded From UniProt	complete
part_of	GO:0055036	virion membrane	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0275	C		Seeded From UniProt	complete
	GO:0044659	cytolysis by virus of host cell	PMID:16030234	ECO:0006006			P		Figure 5 shows the electrochemical assays performed on recombinant E. coli to determine the physiological effects of PRD1 endolysin (pMG118) involved in viral cytolysis. Host cell permeability (K+ and TPP+), cell growth and cell cytolysis (A550) were measured. Fig. 5a exhibited cell growth that steadily increased but never decreased, and variable changes in permeability. There was a peaked influx and efflux of K+, a decrease in TPP+, and steady, increased A550. The absence of endolysin and the presence of holin (pGZ9) failed to rupture cell membrane. Fig. 5e exhibited cytolysis with co-expressed holin and endolysin. Permeability and cell growth increased and peaked, but decreased dramatically below the initial baseline. The initial influx and latter efflux of K+, TPP+, and A550 values displayed how endolysin works to rupture host membrane when simultaneously present with holin in PRD1 for this synergistic effect.	complete
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