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PMID:10931330
| Citation |
Rydman, PS and Bamford, DH (2000) Bacteriophage PRD1 DNA entry uses a viral membrane-associated transglycosylase activity. Mol. Microbiol. 37:356-63 |
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| Abstract |
Amino acid sequence analyses have indicated that the amino-terminal part of bacteriophage PRD1 structural protein P7 carries a conserved transglycosylase domain. We analysed wild-type PRD1 and different mutant particles in zymograms and found a glycolytic activity that was associated with protein P7. This is the first time a putative bacteriophage or plasmid lytic transglycosylase has been shown to have an enzymatic activity. In the absence of protein P7, the phage DNA replication and host cell lysis were delayed. Gene VII of PRD1 is known to encode proteins P7 and P14. In this investigation, the open reading frame coding for P14 was mapped to the 3' end of gene VII. Proteins P7 and P14 probably form a heteromultimeric complex, which is located at the particle vertices and is involved in the early steps of the PRD1 life cycle |
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| Keywords |
Cell Membrane; DNA Replication; DNA, Viral/metabolism; Electrophoresis, Polyacrylamide Gel; Glycosyltransferases/genetics; Glycosyltransferases/metabolism; Peptidoglycan/metabolism; Protein Structure, Tertiary; Tectiviridae/enzymology; Tectiviridae/physiology; Viral Proteins/genetics; Viral Proteins/metabolism; Virus Replication |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0055036: virion membrane |
ECO:0000314: |
C |
Figure 1. |
complete | ||||
| GO:0003796: lysozyme activity |
ECO:0000314: |
F |
Figure 4 |
complete | ||||
| GO:0044659: viral release from host cell by cytolysis |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Figure 4 |
complete | ||||
|
enables |
GO:0003796: lysozyme activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0003796: lysozyme activity |
ECO:0000314: |
F |
Figure 4. |
complete | ||||
| GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism |
ECO:0000315: |
P |
Figure 4 |
complete | ||||
|
enables |
GO:0003796: lysozyme activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0044659: viral release from host cell by cytolysis |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003796: lysozyme activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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