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PMID:11741849

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Citation

Rydman, PS and Bamford, DH (2002) The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane. J. Bacteriol. 184:104-10

Abstract

Bacteriophage PRD1 encodes two proteins (P7 and P15) that are associated with a muralytic activity. Protein P15 is a soluble beta-1,4-N-acetylmuramidase that causes phage-induced host cell lysis. We demonstrate here that P15 is also a structural component of the PRD1 virion and that it is connected to the phage membrane. Small viral membrane proteins P20 and P22 modulate incorporation of P15 into the virion and may connect it to the phage membrane. The principal muralytic protein involved in PRD1 DNA entry seems to be the putative lytic transglycosylase protein P7, as the absence of protein P15 did not delay initiation of phage DNA replication in the virus-host system used. The incorporation of two different lytic enzymes into virions may reflect the broad host range of bacteriophage PRD1.

Links

PubMed PMC134774

Keywords

Bacteriolysis; Bacteriophage PRD1/enzymology; Bacteriophage PRD1/growth & development; DNA Replication; Glycoside Hydrolases/isolation & purification; Glycoside Hydrolases/metabolism; Gram-Negative Bacteria/virology; Muramidase/isolation & purification; Muramidase/metabolism; Viral Matrix Proteins/isolation & purification; Viral Matrix Proteins/metabolism; Viral Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPPRD:CAPSD

GO:0019012: virion

ECO:0000314:

C

Figure 4.

complete
CACAO 8083

BPPRD:CAPSD

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:VP11

GO:0008933: lytic transglycosylase activity

ECO:0000315:

F

Figure 3

complete
CACAO 5663

BPPRD:VP11

GO:0055036: virion membrane

ECO:0000314:

C

Figure 3.

complete
CACAO 5518

BPPRD:VP11

part_of

GO:0055036: virion membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:P2

GO:0019012: virion

ECO:0000314:

C

Figure 4.

complete
CACAO 8081

BPPRD:P2

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:VLYS

GO:0055036: virion membrane

ECO:0000314:

C

Figure 3 "Zymogram analysis revealed the presence of proteins P7 and P15 in both DNA-filled and empty phage membranes"

complete
CACAO 5679

BPPRD:VLYS

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000314:

P

Figure 1a lane 2 (labeled "wt") shows by zymogram analysis that P15 "degraded the peptidoglycan substrate added to the gels and [was] detected as [a] clear zone."

complete
CACAO 5680

BPPRD:VLYS

part_of

GO:0055036: virion membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:VLYS

involved_in

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BPPRD:P5

GO:0019012: virion

ECO:0000314:

C

Figure 4.

complete
CACAO 8086

BPPRD:P5

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:EXLYS

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000314:

P

Figure 1a lane 2 (labeled "wt") shows that P7 "degraded the peptidoglycan substrate added to the gels and [was] detected as [a] clear zone."

complete
CACAO 5681

BPPRD:EXLYS

GO:0055036: virion membrane

ECO:0000314:

C

Figure 3 "Zymogram analysis revealed the presence of proteins P7 and P15 in both DNA-filled and empty phage membranes"

complete
CACAO 5678

BPPRD:EXLYS

involved_in

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BPPRD:EXLYS

part_of

GO:0055036: virion membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:PKG6

GO:0019012: virion

ECO:0000314:

C

Figure 4.

complete
CACAO 8092

BPPRD:PKG6

part_of

GO:0019012: virion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:ENLYS

part_of

GO:0055036: virion membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPRD:ENLYS

involved_in

GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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