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PMID:11741849
| Citation |
Rydman, PS and Bamford, DH (2002) The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane. J. Bacteriol. 184:104-10 |
|---|---|
| Abstract |
Bacteriophage PRD1 encodes two proteins (P7 and P15) that are associated with a muralytic activity. Protein P15 is a soluble beta-1,4-N-acetylmuramidase that causes phage-induced host cell lysis. We demonstrate here that P15 is also a structural component of the PRD1 virion and that it is connected to the phage membrane. Small viral membrane proteins P20 and P22 modulate incorporation of P15 into the virion and may connect it to the phage membrane. The principal muralytic protein involved in PRD1 DNA entry seems to be the putative lytic transglycosylase protein P7, as the absence of protein P15 did not delay initiation of phage DNA replication in the virus-host system used. The incorporation of two different lytic enzymes into virions may reflect the broad host range of bacteriophage PRD1. |
| Links | |
| Keywords |
Bacteriolysis; Bacteriophage PRD1/enzymology; Bacteriophage PRD1/growth & development; DNA Replication; Glycoside Hydrolases/isolation & purification; Glycoside Hydrolases/metabolism; Gram-Negative Bacteria/virology; Muramidase/isolation & purification; Muramidase/metabolism; Viral Matrix Proteins/isolation & purification; Viral Matrix Proteins/metabolism; Viral Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 4. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0008933: lytic transglycosylase activity |
ECO:0000315: |
F |
Figure 3 |
complete | ||||
| GO:0055036: virion membrane |
ECO:0000314: |
C |
Figure 3. |
complete | ||||
|
part_of |
GO:0055036: virion membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 4. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0055036: virion membrane |
ECO:0000314: |
C |
Figure 3 "Zymogram analysis revealed the presence of proteins P7 and P15 in both DNA-filled and empty phage membranes" |
complete | ||||
| GO:0044659: viral release from host cell by cytolysis |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Figure 1a lane 2 (labeled "wt") shows by zymogram analysis that P15 "degraded the peptidoglycan substrate added to the gels and [was] detected as [a] clear zone." |
complete | ||||
|
part_of |
GO:0055036: virion membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 4. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism |
ECO:0000314: |
P |
Figure 1a lane 2 (labeled "wt") shows that P7 "degraded the peptidoglycan substrate added to the gels and [was] detected as [a] clear zone." |
complete | ||||
| GO:0055036: virion membrane |
ECO:0000314: |
C |
Figure 3 "Zymogram analysis revealed the presence of proteins P7 and P15 in both DNA-filled and empty phage membranes" |
complete | ||||
|
involved_in |
GO:0051672: catabolism by organism of cell wall peptidoglycan in other organism |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0055036: virion membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019012: virion |
ECO:0000314: |
C |
Figure 4. |
complete | ||||
|
part_of |
GO:0019012: virion |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0055036: virion membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0044659: obsolete catabolism by organism of cell wall peptidoglycan in other organism |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
See also
References
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