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Cacao

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GO:0016799hydrolase activity, hydrolyzing N-glycosyl compoundsPMID:7925454IDA: Inferred from Direct Assay F
This annotation made on page: BPPRD:ENLYS
By: DFrank (group Team Green A 2018) on 2018-04-14 18:14:26 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Ivanerill2018-05-02 11:46:35 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-05-02 11:44:22 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0033922 peptidoglycan beta-N-acetylmuramidase activity PMID:7925454 IDA: Inferred from Direct Assay F In figure 3, samples of cell walls were combined with the enzyme P15, hydrolyzed with HCl, and chromatographed on a column. Eluted fractions were then tested for radioactivity. Only the sample with [3H]muramicitol was seen only in the tubes with P15 and cell walls, not in any of the control. This meant that P15 bound to [3H]muramicitol and hydrolyzed it, which would lead to cell death. It had previously been discovered that adding p15 to a tube of E. coli would decrease the turbidity of the tube by killing the cells. Figure 3 was just to show the nature of the cells' death. complete
CACAO 13267
on BPPRD:ENLYS
Flagged
Public
Assessment
Ivanerill2018-05-02 11:25:45 CDT

paper asserts that this is a 1,4-beta-N-acetylmuramidase. look up "peptidoglycan beta-N-acetylmuramidase activity" in quickGO and see if it applies to your protein (see what proteins have been annotated with this term)

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original