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User:TNaqvi

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CACAO Phage Hunters 2019

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableLAMBD:TERL2019-03-11 13:57:35 CDTGO:0019073 viral DNA genome packaging (P)PMID:11866517ECO:0007101 southern hybridization evidence used in manual assertion

Researchers wanted to show that gpA (found in the terminase large subunit) helps package phage DNA. A lambda bacteriophage was used in this experiment and various gpA mutants were cut (with a restriction endonuclease) and cloned into this phage; the amount of DNA packaged was determined experimentally by quantifying it in vivo. Table 5 shows how a normal lambda-P1 Sam7 phage with no mutated gpA was able to package 100% of its DNA. But when the same phage contained any type of mutated gpA, the percentage of DNA packaged decreased dramatically.

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