GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|MOUSE:B7ZWM4||Masonale, MichSt14A 15||2014-04-06 08:04:02 CDT||GO:0002544 chronic inflammatory response (P)||PMID:23860501||ECO:0000314 direct assay evidence used in manual assertion|
Figure 1(E) uses immunostaining of DUOX proteins in DOUX K/O mice and W/T mice. DUOX levels are much higher in W/T mice, particularly toward the apical surface of the epithelium, than it is in K/O mice. This shows that over-expression of DUOX is part of the immune/inflammatory response to Helicobacter felis in mice.
|MOUSE:EGR1||Masonale, MichSt14A 15||2014-04-05 11:13:54 CDT||GO:0035176 social behavior (P)||PMID:20921194||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 3 (I and J) shows significantly increased Egr-1 levels when an adult mouse is exposed to a juvenile mouse as opposed to a non-species specific control.
|MOUSE:TLR2||Masonale, MichSt14A 15||2014-04-04 18:19:55 CDT||GO:0002218 activation of innate immune response (P)||PMID:15501775||ECO:0000314 direct assay evidence used in manual assertion|
Figure 4(A) shows the results of a SuperArray blot. The assay shows that there is increased levels of mRNA for cytokines and cytokine receptors in the W/T mice treated with whole Helicobacter pylori as opposed to the control. 4(B and C) shows that TLR2 K/O mice do not produce IL-6 (a cytokine) in the presence of H. pylori, but do produce cytokine in the presence of E. coli. This suggests that TLR2 is the dominant toll-like receptor to activate the innate immune system in response to Helicobacter.
|CHICK:G0W2S9||Masonale, MichSt14A 15||2014-04-03 11:27:03 CDT||GO:0030284 estrogen receptor activity (F)||PMID:22868208||ECO:0000314 direct assay evidence used in manual assertion|
Figure 4B shows LRP2 expression in the kidneys of both hens and roosters. There is greater expression of LRP2 in hen kidneys, suggesting LRP2 is over-expressed in response to estrogen.
|CHICK:G0W2S9||Masonale, MichSt14A 15||2014-04-03 11:27:03 CDT||GO:0030284 estrogen receptor activity (F)||PMID:22868208||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 5:B and C. Western blot shows that LRP2 expression is increased in roosters treated with estrogen. Roosters treated with estrogen and then sacrificed within a 24 hour period (2, 5, 6) had the greatest amount of over-expression. Roosters who were treated with estrogen, and then sacrificed later than 24 hours (sacrifice at 48 hours -3- or 72 hours -4-). Roosters treated with estrogen multiple times (5 and 6) had the most over-expression of LRP2.
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Pages in category "MichSt14A 15"
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