YEAST:RFA1

Species (Taxon ID)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292)
Gene Name(s)	RFA1 (synonyms: BUF2, RPA1)
Protein Name(s)	Replication factor A protein 1 RF-A protein 1 DNA-binding protein BUF2 Replication protein A 69 kDa DNA-binding subunit Single-stranded DNA-binding protein
External Links
UniProt	P22336
EMBL	X59748 M60262 S64901 L22015 BK006935
PIR	S20145
RefSeq	NP_009404.1
PDB	1YNX
PDBsum	1YNX
ProteinModelPortal	P22336
SMR	P22336
BioGrid	31793
DIP	DIP-2512N
IntAct	P22336
MINT	MINT-476157
STRING	4932.YAR007C
MaxQB	P22336
PaxDb	P22336
PeptideAtlas	P22336
EnsemblFungi	[example_ID YAR007C]
GeneID	851266
KEGG	sce:YAR007C
CYGD	YAR007c
SGD	S000000065
eggNOG	COG1599
GeneTree	ENSGT00390000012403
HOGENOM	HOG000162322
InParanoid	P22336
KO	K07466
OMA	VIAFKGC
OrthoDB	EOG71P2KR
BioCyc	YEAST:G3O-28869-MONOMER
Reactome	REACT_233196 REACT_250453
EvolutionaryTrace	P22336
NextBio	968234
PRO	PR:P22336
Proteomes	UP000002311
Genevestigator	P22336
GO	GO:0000781 GO:0000794 GO:0005737 GO:0005829 GO:0005662 GO:0005634 GO:0003690 GO:0046872 GO:0043565 GO:0003697 GO:0006281 GO:0006260 GO:0006265 GO:0006268 GO:0000724 GO:0045184 GO:0030491 GO:0006312 GO:0006289 GO:0016567 GO:0007131 GO:0000722 GO:0007004 GO:0010833
Gene3D	2.40.50.140
InterPro	IPR012340 IPR004365 IPR013955 IPR007199 IPR004591
Pfam	PF04057 PF08646 PF01336
SUPFAM	SSF50249
TIGRFAMs	TIGR00617

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0006281	DNA repair	PMID:7862153^[1]	ECO:0000315			P		Table 3	complete CACAO 2027
	GO:0043934	sporulation	PMID:7862153^[1]	ECO:0000315			P		Table 6	complete CACAO 2028
enables	GO:0003729	mRNA binding	PMID:23222640^[2]	ECO:0007005	high throughput direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0043934	sporulation	PMID:7862153^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	PMID:7862153^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0045184	establishment of protein localization	PMID:2554144^[3]	ECO:0000353	physical interaction evidence used in manual assertion	SGD:S000005256	P		Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:7761422^[4]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0030491	heteroduplex formation	PMID:12226081^[5]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0016567	protein ubiquitination	PMID:18342608^[6]	ECO:0000353	physical interaction evidence used in manual assertion	SGD:S000000662	P		Seeded From UniProt	complete
involved_in	GO:0016567	protein ubiquitination	PMID:18342608^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0010833	telomere maintenance via telomere lengthening	PMID:22354040^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0007131	reciprocal meiotic recombination	PMID:12072452^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0007004	telomere maintenance via telomerase	PMID:2554144^[3]	ECO:0000353	physical interaction evidence used in manual assertion	SGD:S000005256	P		Seeded From UniProt	complete
involved_in	GO:0006312	mitotic recombination	PMID:7969128^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006289	nucleotide-excision repair	PMID:8910442^[10]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	PMID:7969128^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006268	DNA unwinding involved in DNA replication	PMID:2554144^[3]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006265	DNA topological change	PMID:22885009^[11]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006260	DNA replication	PMID:7969128^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0005829	cytosol	PMID:22932476^[12]	ECO:0000314	direct assay evidence used in manual assertion		C	exists_during:(GO:0071456)	Seeded From UniProt	complete
part_of	GO:0005737	cytoplasm	PMID:15282802^[13]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0005662	DNA replication factor A complex	PMID:2554144^[3]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	PMID:22932476^[12]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
enables	GO:0003697	single-stranded DNA binding	PMID:8804316^[14]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0003690	double-stranded DNA binding	PMID:7761422^[4]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
part_of	GO:0000794	condensed nuclear chromosome	PMID:9679065^[15]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0000781	chromosome, telomeric region	PMID:14702040^[16]	ECO:0000315	mutant phenotype evidence used in manual assertion		C		Seeded From UniProt	complete
involved_in	GO:0000724	double-strand break repair via homologous recombination	PMID:9679065^[15]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000001014	P		Seeded From UniProt	complete
involved_in	GO:0000722	telomere maintenance via recombination	PMID:17202155^[17]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000006657	P		Seeded From UniProt	complete
involved_in	GO:0051321	meiotic cell cycle	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	MGI:MGI:1915525 PANTHER:PTN000588618	P		Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 SGD:S000000065	F		Seeded From UniProt	complete
enables	GO:0043047	single-stranded telomeric DNA binding	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 PomBase:SPBC660.13c	F		Seeded From UniProt	complete
involved_in	GO:0007004	telomere maintenance via telomerase	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 PomBase:SPBC660.13c SGD:S000000065	P		Seeded From UniProt	complete
involved_in	GO:0006289	nucleotide-excision repair	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 SGD:S000000065 UniProtKB:P27694	P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 PomBase:SPBC660.13c SGD:S000000065 UniProtKB:P27694 UniProtKB:Q6YZ49	P		Seeded From UniProt	complete
involved_in	GO:0006268	DNA unwinding involved in DNA replication	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 PomBase:SPBC660.13c SGD:S000000065	P		Seeded From UniProt	complete
involved_in	GO:0006261	DNA-dependent DNA replication	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0010173 PANTHER:PTN000588618 UniProtKB:Q9U0J0	P		Seeded From UniProt	complete
part_of	GO:0005662	DNA replication factor A complex	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0010173 PANTHER:PTN000588618 PomBase:SPBC660.13c RGD:1307376 SGD:S000000065 UniProtKB:P27694 UniProtKB:Q10Q08	C		Seeded From UniProt	complete
enables	GO:0003697	single-stranded DNA binding	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0010173 PANTHER:PTN000588618 PomBase:SPBC660.13c RGD:1307376 SGD:S000000065 UniProtKB:P27694	F		Seeded From UniProt	complete
enables	GO:0003684	damaged DNA binding	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 UniProtKB:P27694	F		Seeded From UniProt	complete
part_of	GO:0000784	nuclear chromosome, telomeric region	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 PomBase:SPBC660.13c	C		Seeded From UniProt	complete
involved_in	GO:0000724	double-strand break repair via homologous recombination	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	MGI:MGI:1915525 PANTHER:PTN000588618 SGD:S000000065 UniProtKB:P27694	P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:21873635^[18]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000588618 PomBase:SPBC660.13c UniProtKB:P27694	P		Seeded From UniProt	complete
enables	GO:0003676	nucleic acid binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004365	F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004591 InterPro:IPR007199	F		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004591 InterPro:IPR007199	C		Seeded From UniProt	complete
involved_in	GO:0006260	DNA replication	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004591 InterPro:IPR007199	P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004591	P		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004591	P		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0539 UniProtKB-SubCell:SL-0191	C		Seeded From UniProt	complete
enables	GO:0046872	metal ion binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0479	F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
involved_in	GO:0006260	DNA replication	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0235	P		Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Firmenich, AA et al. (1995) A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Mol. Cell. Biol. 15 1620-31 PubMed GONUTS page
↑ Mitchell, SF et al. (2013) Global analysis of yeast mRNPs. Nat. Struct. Mol. Biol. 20 127-33 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 Brill, SJ & Stillman, B (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342 92-5 PubMed GONUTS page
↑ ^4.0 ^4.1 Singh, KK & Samson, L (1995) Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes. Proc. Natl. Acad. Sci. U.S.A. 92 4907-11 PubMed GONUTS page
↑ Van Komen, S et al. (2002) Functional cross-talk among Rad51, Rad54, and replication protein A in heteroduplex DNA joint formation. J. Biol. Chem. 277 43578-87 PubMed GONUTS page
↑ ^6.0 ^6.1 Davies, AA et al. (2008) Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a. Mol. Cell 29 625-36 PubMed GONUTS page
↑ Luciano, P et al. (2012) RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts. EMBO J. 31 2034-46 PubMed GONUTS page
↑ Soustelle, C et al. (2002) Replication protein A is required for meiotic recombination in Saccharomyces cerevisiae. Genetics 161 535-47 PubMed GONUTS page
↑ ^9.0 ^9.1 ^9.2 Longhese, MP et al. (1994) Replication factor A is required in vivo for DNA replication, repair, and recombination. Mol. Cell. Biol. 14 7884-90 PubMed GONUTS page
↑ He, Z et al. (1996) Assessing the requirements for nucleotide excision repair proteins of Saccharomyces cerevisiae in an in vitro system. J. Biol. Chem. 271 28243-9 PubMed GONUTS page
↑ Cejka, P et al. (2012) Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes. Mol. Cell 47 886-96 PubMed GONUTS page
↑ ^12.0 ^12.1 Dastidar, RG et al. (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2 30 PubMed GONUTS page
↑ Sundin, BA et al. (2004) Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21 793-800 PubMed GONUTS page
↑ Philipova, D et al. (1996) A hierarchy of SSB protomers in replication protein A. Genes Dev. 10 2222-33 PubMed GONUTS page
↑ ^15.0 ^15.1 Gasior, SL et al. (1998) Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes. Genes Dev. 12 2208-21 PubMed GONUTS page
↑ Schramke, V et al. (2004) RPA regulates telomerase action by providing Est1p access to chromosome ends. Nat. Genet. 36 46-54 PubMed GONUTS page
↑ Grandin, N & Charbonneau, M (2007) Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA. Nucleic Acids Res. 35 822-38 PubMed GONUTS page
↑ ^18.00 ^18.01 ^18.02 ^18.03 ^18.04 ^18.05 ^18.06 ^18.07 ^18.08 ^18.09 ^18.10 ^18.11 ^18.12 ^18.13 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:7862153-1] 1.0 ^1.1 ^1.2 ^1.3 Firmenich, AA et al. (1995) A novel allele of Saccharomyces cerevisiae RFA1 that is deficient in recombination and repair and suppressible by RAD52. Mol. Cell. Biol. 15 1620-31 PubMed GONUTS page

[PMID:23222640-2] Mitchell, SF et al. (2013) Global analysis of yeast mRNPs. Nat. Struct. Mol. Biol. 20 127-33 PubMed GONUTS page

[PMID:2554144-3] 3.0 ^3.1 ^3.2 ^3.3 Brill, SJ & Stillman, B (1989) Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication. Nature 342 92-5 PubMed GONUTS page

[PMID:7761422-4] 4.0 ^4.1 Singh, KK & Samson, L (1995) Replication protein A binds to regulatory elements in yeast DNA repair and DNA metabolism genes. Proc. Natl. Acad. Sci. U.S.A. 92 4907-11 PubMed GONUTS page

[PMID:12226081-5] Van Komen, S et al. (2002) Functional cross-talk among Rad51, Rad54, and replication protein A in heteroduplex DNA joint formation. J. Biol. Chem. 277 43578-87 PubMed GONUTS page

[PMID:18342608-6] 6.0 ^6.1 Davies, AA et al. (2008) Activation of ubiquitin-dependent DNA damage bypass is mediated by replication protein a. Mol. Cell 29 625-36 PubMed GONUTS page

[PMID:22354040-7] Luciano, P et al. (2012) RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts. EMBO J. 31 2034-46 PubMed GONUTS page

[PMID:12072452-8] Soustelle, C et al. (2002) Replication protein A is required for meiotic recombination in Saccharomyces cerevisiae. Genetics 161 535-47 PubMed GONUTS page

[PMID:7969128-9] 9.0 ^9.1 ^9.2 Longhese, MP et al. (1994) Replication factor A is required in vivo for DNA replication, repair, and recombination. Mol. Cell. Biol. 14 7884-90 PubMed GONUTS page

[PMID:8910442-10] He, Z et al. (1996) Assessing the requirements for nucleotide excision repair proteins of Saccharomyces cerevisiae in an in vitro system. J. Biol. Chem. 271 28243-9 PubMed GONUTS page

[PMID:22885009-11] Cejka, P et al. (2012) Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes. Mol. Cell 47 886-96 PubMed GONUTS page

[PMID:22932476-12] 12.0 ^12.1 Dastidar, RG et al. (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2 30 PubMed GONUTS page

[PMID:15282802-13] Sundin, BA et al. (2004) Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21 793-800 PubMed GONUTS page

[PMID:8804316-14] Philipova, D et al. (1996) A hierarchy of SSB protomers in replication protein A. Genes Dev. 10 2222-33 PubMed GONUTS page

[PMID:9679065-15] 15.0 ^15.1 Gasior, SL et al. (1998) Rad52 associates with RPA and functions with rad55 and rad57 to assemble meiotic recombination complexes. Genes Dev. 12 2208-21 PubMed GONUTS page

[PMID:14702040-16] Schramke, V et al. (2004) RPA regulates telomerase action by providing Est1p access to chromosome ends. Nat. Genet. 36 46-54 PubMed GONUTS page

[PMID:17202155-17] Grandin, N & Charbonneau, M (2007) Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA. Nucleic Acids Res. 35 822-38 PubMed GONUTS page

[PMID:21873635-18] 18.00 ^18.01 ^18.02 ^18.03 ^18.04 ^18.05 ^18.06 ^18.07 ^18.08 ^18.09 ^18.10 ^18.11 ^18.12 ^18.13 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

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YEAST:RFA1

Contents

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References

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