PMID:17202155

Citation	Grandin, N and Charbonneau, M (2007) Control of the yeast telomeric senescence survival pathways of recombination by the Mec1 and Mec3 DNA damage sensors and RPA. Nucleic Acids Res. 35:822-38
Abstract	Saccharomyces cerevisiae telomerase-negative cells undergo homologous recombination on subtelomeric or TG(1-3) telomeric sequences, thus allowing Type I or Type II post-senescence survival, respectively. Here, we find that the DNA damage sensors, Mec1, Mec3 and Rad24 control Type II recombination, while the Rad9 adaptor protein and the Rad53 and Chk1 effector kinases have no effect on survivor type selection. Therefore, the Mec1 and Mec3 checkpoint complexes control telomeric recombination independently of their roles in generating and amplifying the Mec1-Rad53-Chk1 kinase cascade. rfa1-t11 mutant cells, bearing a mutation in Replication Protein A (RPA) conferring a defect in recruiting Mec1-Ddc2, were also deficient in both types of telomeric recombination. Importantly, expression of an Rfa1-t11-Ddc2 hybrid fusion protein restored checkpoint-dependent arrest, but did not rescue defective telomeric recombination. Therefore, the Rfa1-t11-associated defect in telomeric recombination is not solely due to its failure to recruit Mec1. We have also isolated novel alleles of RFA1 that were deficient in Type I but not in Type II recombination and proficient in checkpoint control. Therefore, the checkpoint and recombination functions of RPA can be genetically separated, as can the RPA-mediated control of the two types of telomeric recombination.
Links	PubMed PMC1807969 Online version:10.1093/nar/gkl1081
Keywords	Adaptor Proteins, Signal Transducing; Binding Sites; Cell Cycle Proteins/genetics; Cell Cycle Proteins/physiology; Cyclin B/metabolism; DNA Damage; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Gene Deletion; Intracellular Signaling Peptides and Proteins; Mutation; Phosphoproteins/genetics; Protein-Serine-Threonine Kinases; RNA/genetics; Rad52 DNA Repair and Recombination Protein/genetics; Rad52 DNA Repair and Recombination Protein/metabolism; Recombinant Fusion Proteins/metabolism; Recombination, Genetic; Replication Protein A/genetics; Replication Protein A/physiology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Telomerase/genetics; Telomere/chemistry; Telomere-Binding Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/physiology
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