User:Syedsa

Phage Hunters Spring 2017

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
flagged	9CAUD:B6SBV8	2017-04-29 17:09:16 CDT	GO:0008745 N-acetylmuramoyl-L-alanine amidase activity (F)	PMID:21803993	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 1a, 1b, and 1c show how the lytic activity and specificity were effected by truncation. Figure 1a goes in depth on how the N- and C-terminal truncations of endolysin CD27L were produced as His-tagged proteins. Figure 1b displays the CD27L (1–179) and the full CD27L both failed to lyse C. tyrobutyricum. Figure 1c gives the assays of 10 the μg Ni-NTA-purified buffer incubated cells.	challenge
flagged	BPPHX:A3FJT0	2017-04-29 23:35:38 CDT	GO:0004857 enzyme inhibitor activity (F)	PMID:28446197	ECO:0000314 direct assay evidence used in manual assertion	Table 3 shows the activity levels of B. licheniformis. The activity percentages show how growth is inhibited by 0.5 µM of the E protein when compared to the normal growth of B. licheniformis after 6 hours. The table shows PduP-E from Ec3087 having the greatest inhibition after induction of 0.1 mM Rha.	challenge

acceptable:0
unacceptable:0
requires_changes:0
flagged:2

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Puglisk1, Team Blue-B	BPPH2:NP	GO:0017053 - transcription repressor complex (C)	PMID:12426390	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2
requireschanges	Stuitjj, Team X-wing@Alliciousness	BPPH2:CAPSD	GO:0019012 - virion (C)	PMID:15837419	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
requireschanges	Khellfa, Team The Lysogenizers	BPB03:HOLIN	GO:0044659 - viral release from host cell by cytolysis (P)	GO_REF:0000100	ECO:0000247 sequence alignment evidence used in manual assertion	challenge	C: 1
acceptable	Mcgillje, Team Phergalicious	BPSPP:O48472	GO:0020002 - host cell plasma membrane (C)	PMID:27825035	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Syedsa