User:Smedleya

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	KOMXY:D2KKE6	2012-04-10 11:58:59 CDT	GO:0006044 N-acetylglucosamine metabolic process (P)	PMID:21655093	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows that when the gene is interrupted, the culture cannot grow on N-acetylglucosamine, showing an interruption in the metabolic pathway.	challenge
requireschanges	XANC5:Q3BYK8	2012-04-14 12:51:34 CDT	GO:0009306 protein secretion (P)	PMID:21576326	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows systematic mutations in the protein sequence that affect virulence via interactions (or lack thereof) with HrcU, a known type III secretion protein	challenge
requireschanges	XANC5:Q3BYK6	2012-04-14 12:50:11 CDT	GO:0009306 protein secretion (P)	PMID:21219463	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that HrcU does not prevent the expression of secreted proteins, but rather prevents the secretion of these proteins when mutated.	challenge
acceptable	RALSO:HRPB	2012-04-14 14:21:40 CDT	GO:0030260 entry into host cell (P)	PMID:10707351	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4 shows that a mutant hrpB cell has reduced entry into a host cell than compared to wild type.	challenge
requireschanges	XANC5:Q3BYL5	2012-04-14 16:30:06 CDT	GO:0009297 pilus assembly (P)	PMID:15774889	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows an immunoblot using antibodies against HrpE to show that the pilus is primarily composed of HrpE.	challenge
unacceptable	XANC5:Q3BYK0	2012-04-15 10:20:24 CDT	GO:0006200 ATP catabolic process (P)	PMID:19114489	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows that the ATP hydrolysis activity of the protein in vitro.	challenge
requireschanges	XANC5:Q3BYK0	2012-04-15 10:20:24 CDT	GO:0009405 pathogenesis (P)	PMID:19114489	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows that when mutated, the host has significantly reduced symptom formation.	challenge
requireschanges	9XANT:Q0VHD4	2012-04-15 14:05:17 CDT	GO:0009405 pathogenesis (P)	PMID:21478322	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows that Hpa2 contributes to pathogenesis of rice plants because it can compliment the double mutant of Hpa2 and HrpF (which is also shown to contribute to lesion formation).	challenge
requireschanges	9XANT:Q0VHD4	2012-04-15 14:18:14 CDT	GO:0072657 protein localization in membrane (P)	PMID:21478322	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows that the Hpa2 protein is localized at the host cell membrane.	challenge
requireschanges	9XANT:Q0VHC0	2012-04-15 14:17:18 CDT	GO:0009405 pathogenesis (P)	PMID:21478322	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1 shows that HrpF1 contributes to pathogenesis of rice plants because it can compliment the double mutant of HrpF1 and Hpa2 (which is also shown to contribute to lesion formation).	challenge
requireschanges	9XANT:Q0VHC0	2012-04-15 14:17:18 CDT	GO:0072657 protein localization in membrane (P)	PMID:21478322	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows that when HrpF1 is able to bind with Hpa2 in plant cells, it is localized in the plant cell membrane.	challenge
requireschanges	XANCP:Q9LCF2	2012-04-15 14:51:11 CDT	GO:0009405 pathogenesis (P)	PMID:19423625	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that XrvA contributes to the pathogenesis but is not completely dependent on it. Figure 5 also shows that XrvA has an influence on the expression levels of other known virulence genes.	challenge

acceptable:1
unacceptable:1
requires_changes:9
flagged:0

Annotations challenged by Smedleya

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Hufnag30, Team The Bioneers	MAIZE:PGMC1	GO:0004614 - phosphoglucomutase activity (F)	PMID:9662542	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Smedleya

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	AZOBR:Q8KXD5	2012-03-27 11:57:23 CDT	GO:0042619 poly-hydroxybutyrate biosynthetic process (P)	PMID:12039753	ECO:0000315 mutant phenotype evidence used in manual assertion	As shown in Figure 6, when grown in excess carbon the normal strain produced polyhydroxybutyrate granules but the mutant did not under the same conditions.	challenge
requireschanges	METEA:C5AZN7	2012-04-01 14:20:19 CDT	GO:0003988 acetyl-CoA C-acyltransferase activity (F)	PMID:11208803	ECO:0000270 expression pattern evidence used in manual assertion	Table 2 shows the beta-ketothiolase activity of the AM1 strain. It also shows the same activity of a phaB mutant and the no activity for a phaA mutant.	challenge
acceptable	PSEPU:B5M4W4	2012-04-01 14:50:28 CDT	GO:0042621 poly(3-hydroxyalkanoate) biosynthetic process (P)	PMID:16085828	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 shows that when phaG is inturrupted, polyhydroxyalkanoate synthesis is inhibited.	challenge

acceptable:2
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Smedleya

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Hufnag30, Team The Bioneers	MAIZE:PGMC1	GO:0004614 - phosphoglucomutase activity (F)	PMID:9662542	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Smedleya