PMID:21655093

Citation	Yadav, V, Panilaitis, B, Shi, H, Numuta, K, Lee, K and Kaplan, DL (2011) N-acetylglucosamine 6-phosphate deacetylase (nagA) is required for N-acetyl glucosamine assimilation in Gluconacetobacter xylinus. PLoS ONE 6:e18099
Abstract	Metabolic pathways for amino sugars (N-acetylglucosamine; GlcNAc and glucosamine; Gln) are essential and remain largely conserved in all three kingdoms of life, i.e., microbes, plants and animals. Upon uptake, in the cytoplasm these amino sugars undergo phosphorylation by phosphokinases and subsequently deacetylation by the enzyme N-acetylglucosamine 6-phosphate deacetylase (nagA) to yield glucosamine-6-phosphate and acetate, the first committed step for both GlcNAc assimilation and amino-sugar-nucleotides biosynthesis. Here we report the cloning of a DNA fragment encoding a partial nagA gene and its implications with regard to amino sugar metabolism in the cellulose producing bacterium Glucoacetobacter xylinus (formally known as Acetobacter xylinum). For this purpose, nagA was disrupted by inserting tetracycline resistant gene (nagA::tet(r); named as ΔnagA) via homologous recombination. When compared to glucose fed conditions, the UDP-GlcNAc synthesis and bacterial growth (due to lack of GlcNAc utilization) was completely inhibited in nagA mutants. Interestingly, that inhibition occured without compromising cellulose production efficiency and its molecular composition under GlcNAc fed conditions. We conclude that nagA plays an essential role for GlcNAc assimilation by G. xylinus thus is required for the growth and survival for the bacterium in presence of GlcNAc as carbon source. Additionally, G. xylinus appears to possess the same molecular machinery for UDP-GlcNAc biosynthesis from GlcNAc precursors as other related bacterial species.
Links	PubMed PMC3107205 Online version:10.1371/journal.pone.0018099
Keywords	Acetylglucosamine/analogs & derivatives; Acetylglucosamine/biosynthesis; Amidohydrolases/genetics; Amidohydrolases/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cloning, Molecular; Cytoplasm/metabolism; Gluconacetobacter xylinus/genetics; Gluconacetobacter xylinus/growth & development; Gluconacetobacter xylinus/metabolism; Microbial Viability; Microscopy, Atomic Force; Molecular Sequence Data; Mutation; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Uridine Diphosphate Sugars/biosynthesis
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
GLUXY:D2KKE6	involved_in	GO:0006044: N-acetylglucosamine metabolic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
KOMXY:D2KKE6		GO:0006044: N-acetylglucosamine metabolic process	ECO:0000315:		P		Figure 4 shows that when the gene is interrupted, the culture cannot grow on N-acetylglucosamine, showing an interruption in the metabolic pathway.	complete CACAO 4301
KOMXY:D2KKE6	involved_in	GO:0006044: N-acetylglucosamine metabolic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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