PMID:21478322

Citation	Li, YR, Che, YZ, Zou, HS, Cui, YP, Guo, W, Zou, LF, Biddle, EM, Yang, CH and Chen, GY (2011) Hpa2 required by HrpF to translocate Xanthomonas oryzae transcriptional activator-like effectors into rice for pathogenicity. Appl. Environ. Microbiol. 77:3809-18
Abstract	Xanthomonas oryzae pv. oryzicola, the causative agent of bacterial leaf streak, injects a plethora of effectors through the type III secretion system (T3SS) into rice cells to cause disease. The T3SS, encoded by the hrp genes, is essential for the pathogen to elicit the hypersensitive response (HR) in nonhost tobacco and for pathogenicity in host rice. Whether or not a putative lytic transglycosylase, Hpa2, interacts with a translocon protein, HrpF, to facilitate bacterial pathogenicity remains unknown. Here we demonstrated that both the hpa2 and hrpF genes are required for the pathogenicity of X. oryzae pv. oryzicola strain RS105 in rice but not for HR induction in tobacco. The expression of hpa2 was positively regulated by HrpG and HrpD6 but not by HrpX. In vivo secretion and subcellular localization analyses confirmed that Hpa2 secretion is dependent on HpaB (a T3SS exit protein) and that Hpa2 binds to the host cell membrane. Protein-protein assays demonstrated that Hpa2 interacts with HrpF. In planta translocation of AvrXa10 indicated that the mutation in hpa2 and hrpF inhibits the injection of the HpaB-dependent transcriptional activator-like (TAL) effector into rice. These findings suggest that Hpa2 and HrpF form a complex to translocate T3S effectors into plant cells for pathogenesis in host rice.
Links	PubMed PMC3127602 Online version:10.1128/AEM.02849-10
Keywords	Bacterial Proteins/metabolism; Glycosyltransferases/metabolism; Oryza sativa/microbiology; Plant Diseases/microbiology; Protein Binding; Protein Interaction Mapping; Protein Transport; Tobacco/microbiology; Trans-Activators/metabolism; Virulence Factors/metabolism; Xanthomonas/metabolism; Xanthomonas/pathogenicity
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9XANT:Q0VHD4		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 1 shows that Hpa2 contributes to pathogenesis of rice plants because it can compliment the double mutant of Hpa2 and HrpF (which is also shown to contribute to lesion formation).	complete CACAO 4900
9XANT:Q0VHD4		GO:0072657: protein localization in membrane	ECO:0000314:		P		Figure 5 shows that the Hpa2 protein is localized at the host cell membrane.	complete CACAO 4902
9XANT:Q0VHC0		GO:0009405: pathogenesis	ECO:0000315:		P		Figure 1 shows that HrpF1 contributes to pathogenesis of rice plants because it can compliment the double mutant of HrpF1 and Hpa2 (which is also shown to contribute to lesion formation).	complete CACAO 4906
9XANT:Q0VHC0		GO:0072657: protein localization in membrane	ECO:0000314:		P		Figure 5 shows that when HrpF1 is able to bind with Hpa2 in plant cells, it is localized in the plant cell membrane.	complete CACAO 4908

References

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