User:Semanchik

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	RHOCA:RNFA	2013-09-13 20:36:33 CDT	GO:0016021 integral to membrane (C)	PMID:9154934	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B illustrates the PhoA activity of diazotrophically grown cells split into chromatophore and soluble fractions. The high PhoA activity in chromatophore fragments and low PhoA activity in soluble fragments suggests that RnfA has alternating hydrophobic and hydrophilic regions; thus, RnfA is a transmembrane protein.	challenge
unacceptable	VIBFM:B5FFH2	2013-09-28 09:12:38 CDT	GO:0045892 negative regulation of transcription, DNA-dependent (P)	PMID:9353217	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows that hns- and hns-rpoS-mutants of MC4110/pW21A and MC4110/pHK555 strains rapidly developed high luminescence levels. In addition, Figure 4 illustrates that both novobiocin and nalidixic-acid, DNA gyrase inhibitors, increase the luminescence of MC4110 rpoS/ptv1073 cells more than 100-fold. Together, these data suggest that the H-NS protein inhibits transcription in E. Coli of the lux systems of all or most luminous bacteria.	challenge
acceptable	RHOCA:RNFA	2013-09-28 09:34:22 CDT	GO:0070111 organellar chromatophore (C)	PMID:9154934	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B illustrates a high amount of alkaline phosphatase activity in the chromatophore fraction of R. capsulatus cells; therefore, a high amount of RnfA activity.	challenge
updatedbyinstructor	HUMAN:TRAP1	2013-10-13 12:53:02 CDT	GO:0009386 translational attenuation (P)	PMID:24113185	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1a: Control cells incorporate less radioactively labeled amino acids compared with TRAP1 KD cells (total lysates brief exposure), suggesting a role for TRAP1 in the global attenuation of translation.	challenge
acceptable	MYCTU:GLND	2013-11-06 16:50:24 CST	GO:0070566 adenylyltransferase activity (F)	PMID:23352854	ECO:0000315 mutant phenotype evidence used in manual assertion	glnD deletion mutant was unable to adenylylate GlnK, while it was adenylylated in the wild type strain (Suppl. Figure S2).	challenge
acceptable	ACEWO:C4N8U0	2013-11-10 16:10:56 CST	GO:0005886 plasma membrane (C)	PMID:19222539	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. Western blot with antisera against RnfC; samples from cell extract and membranes match	challenge
acceptable	ACEWO:C4N8U2	2013-11-10 16:17:54 CST	GO:0005886 plasma membrane (C)	PMID:19222539	ECO:0000314 direct assay evidence used in manual assertion	Figure 3. Western blot with antisera against RnfG yielded identical band patterns for cellular extract and membrane	challenge
requireschanges	SHEON:Q8EG33	2013-11-10 16:39:56 CST	GO:0022904 respiratory electron transport chain (P)	PMID:22360295	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1C: the surface density of cells of the (-)omcA mutant attributable to hematite-specific cell accumulation (A) decreased to 45% of Amax	challenge

acceptable:5
unacceptable:1
requires_changes:1
flagged:0

Annotations challenged by Semanchik

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Delwadia, Team People that mess up	ALIFS:B9UP04	GO:0009927 - histidine phosphotransfer kinase activity (F)	PMID:18441062	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Atruchon
unacceptable	Mustful, Team Bcereus	?		PMID:7592489	IDA: Inferred from Direct Assay	challenge	C: 2

0 annotations fixed by Semanchik

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