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PMID:19222539
| Citation |
Biegel, E, Schmidt, S and Müller, V (2009) Genetic, immunological and biochemical evidence for a Rnf complex in the acetogen Acetobacterium woodii. Environ. Microbiol. 11:1438-43 |
|---|---|
| Abstract |
Acetogenic bacteria grow by the oxidation of various substrates coupled to the reduction of carbon dioxide (acetogenesis) or other electron acceptors but the mechanisms of energy conservation are still enigmatic. Here, we report the presence of a rnf gene cluster rnfCDGEAB in Acetobacterium woodii that is speculated to encode a novel, energy-conserving ferredoxin:NAD(+)-oxidoreductase complex composed of at least six different subunits. Transcriptional analysis revealed that the genes constitute an operon. RnfC and RnfG were heterologously produced and antibodies were generated. Western blot analyses demonstrated that these subunits were produced and are associated with the cytoplasmic membrane. The subunits were present in cells respiring with either carbon dioxide or caffeate. A preparation with NADH dehydrogenase activity was obtained from detergent solubilized membranes that contained RnfC and RnfG. |
| Links |
PubMed Online version:10.1111/j.1462-2920.2009.01871.x |
| Keywords |
Acetobacterium/enzymology; Acetobacterium/genetics; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Blotting, Western; Cell Membrane/chemistry; Clostridium tetani/genetics; Gene Expression; Genes, Bacterial; Nitrate Reductase (NADH)/genetics; Nucleic Acid Amplification Techniques; Operon; Oxidoreductases/genetics; Oxidoreductases/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0005886 : plasma membrane |
ECO:0000314: |
C |
Figure 3. Western blot with antisera against RnfC; samples from cell extract and membranes match |
complete | ||||
| GO:0005886: plasma membrane |
ECO:0000314: |
C |
Figure 3. Western blot with antisera against RnfG yielded identical band patterns for cellular extract and membrane |
complete | ||||
|
part_of |
GO:0005886: plasma membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
See also
References
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